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Literature summary for 1.4.3.19 extracted from
- Purification and properties of glycine oxidase from Pseudomonas putida KT2440 (2015), J. Nutr. Sci. Vitaminol., 61, 506-510 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Pseudomonas putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not inhibited by glycine, sarcosine, and glycine ethyl ester | Pseudomonas putida |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.43 | - |
glycine | at pH 8.5 and 40°C | Pseudomonas putida |  |
| 4.86 | - |
sarcosine | at pH 8.5 and 40°C | Pseudomonas putida | |
| 7.68 | - |
N-ethylglycine | at pH 8.5 and 40°C | Pseudomonas putida | |
| 31.7 | - |
D-proline | at pH 8.5 and 40°C | Pseudomonas putida | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycine + H2O + O2 | Pseudomonas putida | - |
glyoxylate + NH3 + H2O2 | - |
? | |
| glycine + H2O + O2 | Pseudomonas putida KT 2240 | - |
glyoxylate + NH3 + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas putida | Q88Q83 | - |
- |
| Pseudomonas putida KT 2240 | Q88Q83 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-alanine + H2O + O2 | 10% activity compared to glycine | Pseudomonas putida | ? | - |
? | |
| D-alanine + H2O + O2 | 10% activity compared to glycine | Pseudomonas putida KT 2240 | ? | - |
? | |
| D-proline + H2O + O2 | 25% activity compared to glycine | Pseudomonas putida | ? | - |
? | |
| D-proline + H2O + O2 | 25% activity compared to glycine | Pseudomonas putida KT 2240 | ? | - |
? | |
| glycine + H2O + O2 | - |
Pseudomonas putida | glyoxylate + NH3 + H2O2 | - |
? | |
| glycine + H2O + O2 | - |
Pseudomonas putida KT 2240 | glyoxylate + NH3 + H2O2 | - |
? | |
| glycine ethyl ester + H2O + O2 | 5% activity compared to glycine | Pseudomonas putida | ? | - |
? | |
| glycine ethyl ester + H2O + O2 | 5% activity compared to glycine | Pseudomonas putida KT 2240 | ? | - |
? | |
| glycylglycine + H2O + O2 | 3% activity compared to glycine | Pseudomonas putida | ? | - |
? | |
| N-ethylglycine + H2O + O2 | 40% activity compared to glycine | Pseudomonas putida | ? | - |
? | |
| sarcosine + H2O + O2 | - |
Pseudomonas putida | ? | - |
? | |
| sarcosine + H2O + O2 | - |
Pseudomonas putida KT 2240 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | - |
Pseudomonas putida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ThiO | - |
Pseudomonas putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Pseudomonas putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Pseudomonas putida |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 10 | the enzyme is most stable in the pH range from 8.0 to 10.0 upon incubation at 25°C for 1 h | Pseudomonas putida |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Pseudomonas putida | |
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