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Literature summary for 1.4.3.19 extracted from

  • Yao, P.; Lin, Y.; Wu, G.; Lu, Y.; Zhan, T.; Kumar, A.; Zhang, L.; Liu, Z.
    Improvement of glycine oxidase by DNA shuffling, and site-saturation mutagenesis of F247 residue (2015), Int. J. Biol. Macromol., 79, 965-970 .
    View publication on PubMed
Search for more literature for 1.4.3.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus cereus

Protein Variants

Protein Variants Comment Organism
F247P inactive Bacillus cereus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-iminoacetate + H2O Bacillus cereus
-
 glyoxylate + NH3
-
 ?
glycine + H2O + O2 Bacillus cereus
-
 glyoxylate + NH3 + H2O2
-
 ?
glycine + O2 Bacillus cereus
-
 2-iminoacetate + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus cereus L7T8Y9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-iminoacetate + H2O
-
 Bacillus cereus glyoxylate + NH3
-
 ?
glycine + H2O + O2
-
 Bacillus cereus glyoxylate + NH3 + H2O2
-
 ?
glycine + O2
-
 Bacillus cereus 2-iminoacetate + H2O2
-
 ?
sarcosine + H2O + O2
-
 Bacillus cereus glyoxylate + methylamine + H2O2
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Bacillus cereus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
 Bacillus cereus