Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus cereus |
Protein Variants | Comment | Organism |
---|---|---|
N336A | the mutant shows increased substrate affinity compared to the wild type enzyme | Bacillus cereus |
N336G | the mutant shows increased substrate affinity compared to the wild type enzyme | Bacillus cereus |
N336H | the mutant shows increased substrate affinity compared to the wild type enzyme | Bacillus cereus |
N336K | the mutant shows increased substrate affinity compared to the wild type enzyme | Bacillus cereus |
N336R | the mutant shows increased substrate affinity compared to the wild type enzyme | Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.53 | - |
glycine | mutant enzyme N336R, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
0.79 | - |
glycine | mutant enzyme N336K, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
0.95 | - |
glycine | mutant enzyme N336H, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
1.04 | - |
glycine | wild type enzyme, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
1.41 | - |
glycine | mutant enzyme N336A, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
1.51 | - |
sarcosine | wild type enzyme, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
2.25 | - |
glycine | mutant enzyme N336G, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
10.44 | - |
glyphosate | mutant enzyme N336R, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
22.45 | - |
glyphosate | mutant enzyme N336K, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
34.65 | - |
D-alanine | wild type enzyme, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
42.31 | - |
glyphosate | mutant enzyme N336H, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
68.36 | - |
glyphosate | mutant enzyme N336A, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
84.79 | - |
glyphosate | wild type enzyme, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
96.73 | - |
glyphosate | mutant enzyme N336G, at pH 7.5, temperature not specified in the publication | Bacillus cereus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycine + H2O + O2 | Bacillus cereus | - |
glyoxylate + NH3 + H2O2 | - |
? | |
glycine + H2O + O2 | Bacillus cereus HYC-7 | - |
glyoxylate + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | L7T8Y9 | - |
- |
Bacillus cereus HYC-7 | L7T8Y9 | - |
- |
Purification (Comment) | Organism |
---|---|
GST-Bind resin column chromatography and Superdex 200 gel filtration | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-alanine + H2O + O2 | - |
Bacillus cereus | ? | - |
? | |
D-alanine + H2O + O2 | - |
Bacillus cereus HYC-7 | ? | - |
? | |
glycine + H2O + O2 | - |
Bacillus cereus | glyoxylate + NH3 + H2O2 | - |
? | |
glycine + H2O + O2 | - |
Bacillus cereus HYC-7 | glyoxylate + NH3 + H2O2 | - |
? | |
glyphosate + H2O + O2 | - |
Bacillus cereus | ? | - |
? | |
glyphosate + H2O + O2 | - |
Bacillus cereus HYC-7 | ? | - |
? | |
sarcosine + H2O + O2 | - |
Bacillus cereus | ? | - |
? | |
sarcosine + H2O + O2 | - |
Bacillus cereus HYC-7 | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
glyphosate | mutant enzyme N336R, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
0.024 | - |
glycine | mutant enzyme N336R, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
0.19 | - |
glyphosate | mutant enzyme N336K, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
0.23 | - |
glyphosate | mutant enzyme N336H, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
0.25 | - |
glycine | mutant enzyme N336K, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
0.37 | - |
glycine | mutant enzyme N336H, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
0.52 | - |
glycine | mutant enzyme N336A, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
0.62 | - |
glyphosate | mutant enzyme N336G, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
0.67 | - |
glyphosate | mutant enzyme N336A, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
0.67 | - |
glycine | mutant enzyme N336G, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
0.71 | - |
glycine | wild type enzyme, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
0.81 | - |
D-alanine | wild type enzyme, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
0.87 | - |
glyphosate | wild type enzyme, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
0.98 | - |
sarcosine | wild type enzyme, at pH 7.5, temperature not specified in the publication | Bacillus cereus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
glyphosate | mutant enzyme N336R, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
0.0054 | - |
glyphosate | mutant enzyme N336H, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
0.0064 | - |
glyphosate | mutant enzyme N336G, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
0.0084 | - |
glyphosate | mutant enzyme N336K, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
0.0098 | - |
glyphosate | mutant enzyme N336A, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
0.01 | - |
glyphosate | wild type enzyme, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
0.023 | - |
D-alanine | wild type enzyme, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
0.045 | - |
glycine | mutant enzyme N336R, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
0.3 | - |
glycine | mutant enzyme N336G, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
0.32 | - |
glycine | mutant enzyme N336K, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
0.37 | - |
glycine | mutant enzyme N336A, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
0.39 | - |
glycine | mutant enzyme N336H, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
0.65 | - |
sarcosine | wild type enzyme, at pH 7.5, temperature not specified in the publication | Bacillus cereus | |
0.68 | - |
glycine | wild type enzyme, at pH 7.5, temperature not specified in the publication | Bacillus cereus |