Application | Comment | Organism |
---|---|---|
biotechnology | StLASPO represents an appropriate biocatalyst for the resolution of racemic solutions of D,L-aspartate and a well-suited protein scaffold to evolve a L-amino acid oxidase activity by protein engineering | Sulfurisphaera tokodaii |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Sulfurisphaera tokodaii |
expression in Escherichia coli in the active form as holoenzyme | Sulfurisphaera tokodaii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-Aspartate | weak inhibition; weak inhibition | Sulfurisphaera tokodaii | |
oxaloacetate | weak inhibition; weak inhibition | Sulfurisphaera tokodaii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
O2 | pH 8, 37°C | Sulfurisphaera tokodaii | |
1.3 | - |
L-aspartate | apparent value, at pH 8.0 and 37°C | Sulfurisphaera tokodaii | |
13.3 | - |
L-aspartate | pH 8, 37°C | Sulfurisphaera tokodaii | |
18.1 | - |
L-asparagine | apparent value, at pH 8.0 and 37°C | Sulfurisphaera tokodaii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
gel filtration | Sulfurisphaera tokodaii |
53647 | - |
1 * 53647, calculated from amino acid sequence | Sulfurisphaera tokodaii |
536467 | - |
x * 536467, calculated from sequence | Sulfurisphaera tokodaii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O + O2 | Sulfurisphaera tokodaii | - |
4-amino-2,4-dioxobutanoate + NH3 + H2O2 | - |
? | |
L-aspartate + H2O + O2 | Sulfurisphaera tokodaii | the enzyme oxidizes L-aspartate to iminosuccinate, which is then non-enzymatically hydrolyzed to oxaloacetate | oxaloacetate + NH3 + H2O2 | - |
? | |
additional information | Sulfurisphaera tokodaii | the enzyme is absolutely stereoselective, since no activity is detected on D-aspartate. The enzyme shows no activity with L-phenylalanine, L-glutamate, glycine, L-proline, and L-alanine | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurisphaera tokodaii | KC333624 | synthetic construct | - |
Sulfurisphaera tokodaii | Q972D2 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfurisphaera tokodaii |
HiTrap nickel chelating affinity column chromatography, and Superdex 200 gel filtration | Sulfurisphaera tokodaii |
Storage Stability | Organism |
---|---|
4°, purified enzyme at pH 7.5, several months, nploss of activity | Sulfurisphaera tokodaii |
4°C, pH 7.5, in the dark, stable for months | Sulfurisphaera tokodaii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-asparagine + H2O + O2 | - |
Sulfurisphaera tokodaii | 4-amino-2,4-dioxobutanoate + NH3 + H2O2 | - |
? | |
L-asparagine + O2 | Vmax/Km is 63fold lower compared to L-aspartate | Sulfurisphaera tokodaii | 4-amino-2-imino-4-oxobutanoate + H2O2 | - |
? | |
L-aspartate + H2O + O2 | the enzyme oxidizes L-aspartate to iminosuccinate, which is then non-enzymatically hydrolyzed to oxaloacetate | Sulfurisphaera tokodaii | oxaloacetate + NH3 + H2O2 | - |
? | |
L-aspartate + O2 | the enzyme is absolutely stereoselective, since no activity is detected on D-aspartate | Sulfurisphaera tokodaii | iminosuccinate + H2O2 | - |
? | |
additional information | the enzyme does not show activity on L-phenylalanine (50100 mM), L-glutamate (50100 mM), glycine (50100 mM), L-proline (50100 mM) and L-alanine (50100 mM) | Sulfurisphaera tokodaii | ? | - |
? | |
additional information | the enzyme is absolutely stereoselective, since no activity is detected on D-aspartate. The enzyme shows no activity with L-phenylalanine, L-glutamate, glycine, L-proline, and L-alanine | Sulfurisphaera tokodaii | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 536467, calculated from sequence | Sulfurisphaera tokodaii |
monomer | 1 * 53647, calculated from amino acid sequence | Sulfurisphaera tokodaii |
Synonyms | Comment | Organism |
---|---|---|
LASPO | - |
Sulfurisphaera tokodaii |
StLASPO | - |
Sulfurisphaera tokodaii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sulfurisphaera tokodaii |
60 | 80 | - |
Sulfurisphaera tokodaii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 95 | 40°C: about 50% of maximal activity, 95°C: about 60% of maximal activity | Sulfurisphaera tokodaii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
more than 70% of the initial activity is recovered after 60 min at 37°C | Sulfurisphaera tokodaii |
80 | - |
stable up to | Sulfurisphaera tokodaii |
80 | - |
the enzyme is fully stable up to 80°C (400 min). When the temperature is increased to above 85°C, a sharp decrease in enzyme stability is apparent and the activity is lost in about 100 min at 100°C | Sulfurisphaera tokodaii |
83 | - |
Tm-value determinedv by fluoresecence at 340 nm | Sulfurisphaera tokodaii |
86 | - |
Tm-value determinedv by fluoresecence at 525 nm | Sulfurisphaera tokodaii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.05 | - |
L-aspartate | pH 8, 37°C | Sulfurisphaera tokodaii | |
1.05 | - |
L-aspartate | apparent value, at pH 8.0 and 37°C | Sulfurisphaera tokodaii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Sulfurisphaera tokodaii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8.8 | 12 | pH 8.8: about 50% of maximal activity, pH 12.0: about 70% of maximal activity | Sulfurisphaera tokodaii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | 60 min, stable | Sulfurisphaera tokodaii |
7 | 10 | no significant change in activity is observed up to 400 min of incubation at pH 7.5. More than 70% of the initial activity is recovered after 60 min at 37°C. Below pH 8.0 and above pH 10.0, a significant time-dependent inactivation is apparent | Sulfurisphaera tokodaii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | FAD-containing flavoprotein, tight binding of the FAD cofactor | Sulfurisphaera tokodaii | |
FAD | contains 1 FAD per protein monomer | Sulfurisphaera tokodaii |