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Literature summary for 1.4.3.15 extracted from
- Studies on the metabolism of D-amino acid in microorganisms. Part 4. On the nature of the purified D-glutamic-aspartic oxidase of Aspergillus ustus (1957), J. Gen. Appl. Microbiol., 3, 233-239.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| very labile when FAD is removed from the enzyme | Aspergillus ustus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| FMN | competes with FAD | Aspergillus ustus |  |
| KCN | remarkable inhibition at 1 mM, inhibition is only slight with 2,6-dichlorophenol-indophenol electron acceptor but higher when indophenol is used | Aspergillus ustus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-aspartic acid + H2O + O2 | Aspergillus ustus | - |
oxaloacetate + NH3 + H2O2 | - |
? | |
| D-glutamic acid + H2O + O2 | Aspergillus ustus | - |
2-oxoglutarate + NH3 + H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus ustus | - |
strain f | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 90% pure | Aspergillus ustus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| mycelium | - |
Aspergillus ustus | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, several months | Aspergillus ustus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-aspartic acid + H2O + O2 | - |
Aspergillus ustus | oxaloacetate + NH3 + H2O2 | - |
? | |
| D-aspartic acid + H2O + O2 | D-glutamate and D-aspartate are oxidized at the same rate | Aspergillus ustus | oxaloacetate + NH3 + H2O2 | - |
? | |
| D-glutamic acid + H2O + O2 | - |
Aspergillus ustus | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
| D-glutamic acid + H2O + O2 | D-glutamate and D-aspartate are oxidized at the same rate | Aspergillus ustus | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
| additional information | DL-alpha-aminoadipic acid not deaminated, alpha-aminomalonic acid not deaminated, possible acceptors: oxygen, ferricyanide, 2,6-dichlorophenol-indophenol and methylene blue | Aspergillus ustus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Aspergillus ustus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0833 | - |
D-glutamate | - |
Aspergillus ustus | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | flavoprotein | Aspergillus ustus | |
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Release 2023.1 (January 2023)
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