Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.3.14 extracted from

  • Tani, Y.; Omatsu, K.; Saito, S.; Miyake, R.; Kawabata, H.; Ueda, M.; Mihara, H.
    Heterologous expression of l-lysine alpha-oxidase from Scomber japonicus in Pichia pastoris and functional characterization of the recombinant enzyme (2015), J. Biochem., 157, 201-210 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris X-33 cells Scomber japonicus

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 15% residual activity at 1 mM Scomber japonicus
Cu2+ 59% residual activity at 1 mM Scomber japonicus
additional information EDTA, iodoacetamide and iodoacetate show no substantial effects on the enzyme activity Scomber japonicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
L-lysine at pH 7.5 and 70°C Scomber japonicus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information not influenced by Ca2+, Ni2+, and Mg2+ Scomber japonicus
Zn2+ 111% activity at 1 mM Scomber japonicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine + O2 + H2O Scomber japonicus
-
6-amino-2-oxohexanoate + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Scomber japonicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Scomber japonicus

Purification (Commentary)

Purification (Comment) Organism
DEAE-Toyopearl 650M column chromatography and butyl-Toyopearl 650M column chromatography Scomber japonicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-hydroxy-DL-lysine + O2 + H2O 5% activity compared to L-lysine Scomber japonicus 6-amino-5-hydroxy-2-oxohexanoate + NH3 + H2O2
-
?
L-lysine + O2 + H2O
-
Scomber japonicus 6-amino-2-oxohexanoate + NH3 + H2O2
-
?
L-lysine + O2 + H2O 100% activity Scomber japonicus 6-amino-2-oxohexanoate + NH3 + H2O2
-
?
L-ornithine + O2 + H2O 6% activity compared to L-lysine Scomber japonicus 5-amino-2-oxopentanoic acid + NH3 + H2O2
-
?
additional information the enzyme shows no activity with L-histidine, L-aspartic acid, glycine, L-phenylalanine, L-alanine, L-arginine, L-glutamic acid, L-isoleucine, L-leucine, L-serine, L-proline, L-threonine, L-glutamine, L-asparagine, L-threonine, L-tryptophan, L-tyrosine, L-valine, L-methionine, L-cysteine and D-lysine Scomber japonicus ?
-
?

Subunits

Subunits Comment Organism
? x * 69000, SDS-PAGE Scomber japonicus

Synonyms

Synonyms Comment Organism
AIP
-
Scomber japonicus
apoptosis-inducing protein
-
Scomber japonicus
L-lysine alpha-oxidase
-
Scomber japonicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Scomber japonicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 the enzyme is stable below 60°C for at least 1 h Scomber japonicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
203
-
L-lysine at pH 7.5 and 70°C Scomber japonicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Scomber japonicus

pH Range

pH Minimum pH Maximum Comment Organism
4.5 10 the enzyme shows more than 70% of the maximal activity between pH 4.5 and 10.0 Scomber japonicus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10 when the enzyme is incubated at 25°C for 24 h, more than 65% of the original activity remains at pH ranging from 4.0 to 10.0 Scomber japonicus