Cloned (Comment) | Organism |
---|---|
expressed in Pichia pastoris X-33 cells | Scomber japonicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 15% residual activity at 1 mM | Scomber japonicus | |
Cu2+ | 59% residual activity at 1 mM | Scomber japonicus | |
additional information | EDTA, iodoacetamide and iodoacetate show no substantial effects on the enzyme activity | Scomber japonicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
L-lysine | at pH 7.5 and 70°C | Scomber japonicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not influenced by Ca2+, Ni2+, and Mg2+ | Scomber japonicus | |
Zn2+ | 111% activity at 1 mM | Scomber japonicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + O2 + H2O | Scomber japonicus | - |
6-amino-2-oxohexanoate + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Scomber japonicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Scomber japonicus |
Purification (Comment) | Organism |
---|---|
DEAE-Toyopearl 650M column chromatography and butyl-Toyopearl 650M column chromatography | Scomber japonicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-hydroxy-DL-lysine + O2 + H2O | 5% activity compared to L-lysine | Scomber japonicus | 6-amino-5-hydroxy-2-oxohexanoate + NH3 + H2O2 | - |
? | |
L-lysine + O2 + H2O | - |
Scomber japonicus | 6-amino-2-oxohexanoate + NH3 + H2O2 | - |
? | |
L-lysine + O2 + H2O | 100% activity | Scomber japonicus | 6-amino-2-oxohexanoate + NH3 + H2O2 | - |
? | |
L-ornithine + O2 + H2O | 6% activity compared to L-lysine | Scomber japonicus | 5-amino-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
additional information | the enzyme shows no activity with L-histidine, L-aspartic acid, glycine, L-phenylalanine, L-alanine, L-arginine, L-glutamic acid, L-isoleucine, L-leucine, L-serine, L-proline, L-threonine, L-glutamine, L-asparagine, L-threonine, L-tryptophan, L-tyrosine, L-valine, L-methionine, L-cysteine and D-lysine | Scomber japonicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 69000, SDS-PAGE | Scomber japonicus |
Synonyms | Comment | Organism |
---|---|---|
AIP | - |
Scomber japonicus |
apoptosis-inducing protein | - |
Scomber japonicus |
L-lysine alpha-oxidase | - |
Scomber japonicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Scomber japonicus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | the enzyme is stable below 60°C for at least 1 h | Scomber japonicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
203 | - |
L-lysine | at pH 7.5 and 70°C | Scomber japonicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Scomber japonicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 10 | the enzyme shows more than 70% of the maximal activity between pH 4.5 and 10.0 | Scomber japonicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | when the enzyme is incubated at 25°C for 24 h, more than 65% of the original activity remains at pH ranging from 4.0 to 10.0 | Scomber japonicus |