Application | Comment | Organism |
---|---|---|
medicine | antitumor activity | Trichoderma viride |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | - |
Trichoderma viride | |
p-chloromercuribenzoate | - |
Trichoderma viride |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
L-lysine | - |
Trichoderma viride | |
0.044 | - |
L-ornithine | - |
Trichoderma viride | |
1.6 | - |
O2 | - |
Trichoderma viride | |
14 | - |
L-phenylalanine | - |
Trichoderma viride |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Trichoderma viride | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
2 * 56000, SDS-PAGE | Trichoderma viride |
112000 | 119000 | gel filtration, sedimentation equilibrium centrifugation | Trichoderma viride |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-lysine + O2 + H2O | Trichoderma viride | also other alpha,omega-diaminomonocarboxylates and L-lysine hydroxamate are oxidized | 6-amino-2-oxohexanoate + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichoderma viride | - |
mold, grown in wheat bran culture | - |
Trichoderma viride | - |
Y244-2, ATCC 20536 | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, ion-exchange, gel-filtration | Trichoderma viride |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
1.2 U/ml | Trichoderma viride |
66.15 | - |
- |
Trichoderma viride |
Storage Stability | Organism |
---|---|
-20°C, 0.1 M potassium phosphate buffer, pH 7.4, at least 3 months, with little loss of activity | Trichoderma viride |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-arginine + O2 + H2O | - |
Trichoderma viride | 5-guanidino-2-oxopentanoate + NH3 + H2O2 | - |
? | |
L-histidine + O2 + H2O | - |
Trichoderma viride | 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2 | - |
? | |
L-lysine + O2 + H2O | also other alpha,omega-diaminomonocarboxylates and L-lysine hydroxamate are oxidized | Trichoderma viride | 6-amino-2-oxohexanoate + NH3 + H2O2 | - |
? | |
L-ornithine + O2 + H2O | - |
Trichoderma viride | 5-amino-2-oxopentanoic acid + NH3 + H2O2 | - |
? | |
L-phenylalanine + O2 + H2O | - |
Trichoderma viride | phenylpyruvate + NH3 + H2O2 | - |
? | |
L-tyrosine + O2 + H2O | - |
Trichoderma viride | 3-(4-hydroxyphenyl)pyruvate + NH3 + H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 56000, SDS-PAGE | Trichoderma viride |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Trichoderma viride |
50 | - |
for L-lysine | Trichoderma viride |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | 82% relative activity at 30°C, 103% at 50°C and 58°C at 70°C | Trichoderma viride |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
heat labile | Trichoderma viride |
45 | - |
stable for 30 min in the pH range from pH 5.0 or pH 7.0 to pH 10.0 | Trichoderma viride |
55 | - |
0.1 M potassium phosphate buffer, pH 7.4, 30 min, 100% of original activity | Trichoderma viride |
60 | - |
0.1 M potassium phosphate buffer, pH 7.4, 30 min, 92% of original activity | Trichoderma viride |
65 | - |
0.1 M potassium phosphate buffer, pH 7.4, 30 min, 42% of original activity | Trichoderma viride |
70 | - |
0.1 M potassium phosphate buffer, pH 7.4, 30 min, 4% of original activity | Trichoderma viride |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | 9.2 | for L-lysine, reaction rate declines markedly below pH 7.0 | Trichoderma viride |
8 | - |
assay at | Trichoderma viride |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 2 mol per mol of enzyme, prosthetic group | Trichoderma viride |