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Literature summary for 1.4.3.14 extracted from

  • Kusakabe, H.; Kodama, K.; Kuninaka, A.; Yoshino, H.; Misono, H.; Soda, K.
    A new antitumor enzyme, L-lysine alpha-oxidase from Trichoderma viride. Purification and enzymological properties (1980), J. Biol. Chem., 255, 976-981.
    View publication on PubMed

Application

Application Comment Organism
medicine antitumor activity Trichoderma viride

Inhibitors

Inhibitors Comment Organism Structure
HgCl2
-
Trichoderma viride
p-chloromercuribenzoate
-
Trichoderma viride

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
L-lysine
-
Trichoderma viride
0.044
-
L-ornithine
-
Trichoderma viride
1.6
-
O2
-
Trichoderma viride
14
-
L-phenylalanine
-
Trichoderma viride

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Trichoderma viride
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
2 * 56000, SDS-PAGE Trichoderma viride
112000 119000 gel filtration, sedimentation equilibrium centrifugation Trichoderma viride

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-lysine + O2 + H2O Trichoderma viride also other alpha,omega-diaminomonocarboxylates and L-lysine hydroxamate are oxidized 6-amino-2-oxohexanoate + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Trichoderma viride
-
mold, grown in wheat bran culture
-
Trichoderma viride
-
Y244-2, ATCC 20536
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, ion-exchange, gel-filtration Trichoderma viride

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
1.2 U/ml Trichoderma viride
66.15
-
-
Trichoderma viride

Storage Stability

Storage Stability Organism
-20°C, 0.1 M potassium phosphate buffer, pH 7.4, at least 3 months, with little loss of activity Trichoderma viride

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-arginine + O2 + H2O
-
Trichoderma viride 5-guanidino-2-oxopentanoate + NH3 + H2O2
-
?
L-histidine + O2 + H2O
-
Trichoderma viride 3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + H2O2
-
?
L-lysine + O2 + H2O also other alpha,omega-diaminomonocarboxylates and L-lysine hydroxamate are oxidized Trichoderma viride 6-amino-2-oxohexanoate + NH3 + H2O2
-
?
L-ornithine + O2 + H2O
-
Trichoderma viride 5-amino-2-oxopentanoic acid + NH3 + H2O2
-
?
L-phenylalanine + O2 + H2O
-
Trichoderma viride phenylpyruvate + NH3 + H2O2
-
?
L-tyrosine + O2 + H2O
-
Trichoderma viride 3-(4-hydroxyphenyl)pyruvate + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
dimer 2 * 56000, SDS-PAGE Trichoderma viride

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Trichoderma viride
50
-
for L-lysine Trichoderma viride

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 70 82% relative activity at 30°C, 103% at 50°C and 58°C at 70°C Trichoderma viride

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
heat labile Trichoderma viride
45
-
stable for 30 min in the pH range from pH 5.0 or pH 7.0 to pH 10.0 Trichoderma viride
55
-
0.1 M potassium phosphate buffer, pH 7.4, 30 min, 100% of original activity Trichoderma viride
60
-
0.1 M potassium phosphate buffer, pH 7.4, 30 min, 92% of original activity Trichoderma viride
65
-
0.1 M potassium phosphate buffer, pH 7.4, 30 min, 42% of original activity Trichoderma viride
70
-
0.1 M potassium phosphate buffer, pH 7.4, 30 min, 4% of original activity Trichoderma viride

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4 9.2 for L-lysine, reaction rate declines markedly below pH 7.0 Trichoderma viride
8
-
assay at Trichoderma viride

Cofactor

Cofactor Comment Organism Structure
FAD 2 mol per mol of enzyme, prosthetic group Trichoderma viride