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Literature summary for 1.4.3.13 extracted from

  • Giampuzzi, M.; Oleggini, R.; Di Donato, A.
    Demonstration of in vitro interaction between tumor suppressor lysyl oxidase and histones H1 and H2: definition of the regions involved (2003), Biochim. Biophys. Acta, 1647, 245-251.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of mature enzyme and C-terminally deleted enzyme as N-terminally His6-tagged enzymes Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics of the interaction with elastin and histones for recombinant mature enzyme and C-terminally deleted enzyme form Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
18000
-
x * 34000, recombinant mature LOX, SDS-PAGE, x * 18000, recombinant C-terminally deleted LOX, SDS-PAGE Homo sapiens
34000
-
x * 34000, recombinant mature LOX, SDS-PAGE, x * 18000, recombinant C-terminally deleted LOX, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptidyl-L-lysyl-peptide + O2 + H2O Homo sapiens key enzyme in formation of lysine cross-links in side collagen and elastin molecules peptidyl-allysyl-peptide + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification activation of the proenzyme by cleavage, enzyme contains a BMP-1 cleavage site Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged mature enzyme and C-terminally deleted enzyme by nickel affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
histone H1 + O2 + H2O recombinant mature LOX and C-terminally deleted enzyme Homo sapiens ?
-
?
histone H2 + O2 + H2O recombinant mature LOX and C-terminally deleted enzyme Homo sapiens ?
-
?
peptidyl-L-lysyl-peptide + O2 + H2O key enzyme in formation of lysine cross-links in side collagen and elastin molecules Homo sapiens peptidyl-allysyl-peptide + NH3 + H2O2
-
?
peptidyl-L-lysyl-peptide + O2 + H2O in collagen and elastin fibrills Homo sapiens peptidyl-allysyl-peptide + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 34000, recombinant mature LOX, SDS-PAGE, x * 18000, recombinant C-terminally deleted LOX, SDS-PAGE Homo sapiens
More domain structure, overview Homo sapiens

Synonyms

Synonyms Comment Organism
LOX
-
Homo sapiens
lysyl oxidase
-
Homo sapiens
protein-6-oxidase
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
additional information enzyme contains a cross-linked quinone, cross-linking via lysyl- and tyrosyl residues Homo sapiens