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Literature summary for 1.4.3.11 extracted from

  • Yano, Y.; Matsuo, S.; Ito, N.; Tamura, T.; Kusakabe, H.; Inagaki, K.; Imada, K.
    A new L-arginine oxidase engineered from L-glutamate oxidase (2021), Protein Sci., 30, 1044-1055 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.3.11

Application

Application Comment Organism
diagnostics the R305E mutant enzyme is suitable for the determination of L-arginine. L-Arginine can be a medical biomarker because patients with argininemia or cancer show unusual concentrations of L-arginine in the blood Streptomyces sp. X-119-6

Protein Variants

Protein Variants Comment Organism
R305E wild-type enzyme shows strict substrate specificity for L-glutamate. The mutant variant of L-glutamate oxidase exhibits strict specificity for L-arginine. R305E is a thermostable and pH stable enzyme Streptomyces sp. X-119-6

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + O2 + H2O Streptomyces sp. X-119-6
-
 2-oxoglutarate + NH3 + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces sp. X-119-6 Q8L3C7
-
-

Purification (Commentary)

Purification (Comment) Organism
R305E mutant enzyme Streptomyces sp. X-119-6

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + O2 + H2O
-
 Streptomyces sp. X-119-6 2-oxoglutarate + NH3 + H2O2
-
 ?
L-glutamate + O2 + H2O wild-type enzyme shows strict substrate specificity for L-glutamate. The R305E mutant variant of L-glutamate oxidase exhibits strict specificity for L-arginine Streptomyces sp. X-119-6 2-oxoglutarate + NH3 + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
LGOX
-
 Streptomyces sp. X-119-6