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Literature summary for 1.4.3.11 extracted from

  • YaPing, W.; Ben, R.; Hong, Y.; Rui, H.; Li, L.; Pingan, L.; Lixin, M.
    High-level expression of L-glutamate oxidase in Pichia pastoris using multi-copy expression strains and high cell density cultivation (2017), Protein Expr. Purif., 129, 108-114 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris GS115 cells Streptomyces viridosporus

Inhibitors

Inhibitors Comment Organism Structure
Cr2+ about 90% residual activity at 1 mM Streptomyces viridosporus
Cu2+ less than 40% residual activity at 1 mM Streptomyces viridosporus
Fe3+ about 42% residual activity at 1 mM Streptomyces viridosporus
Mn2+ about 65% residual activity at 1 mM Streptomyces viridosporus
Zn2+ about 25% residual activity at 1 mM Streptomyces viridosporus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ about 115% activity at 1 mM Streptomyces viridosporus
additional information not influenced by Li+, Ca2+, and Co2+ Streptomyces viridosporus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + O2 + H2O Streptomyces viridosporus
-
2-oxoglutarate + NH3 + H2O2
-
?
L-glutamate + O2 + H2O Streptomyces viridosporus ATCC 14672
-
2-oxoglutarate + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces viridosporus D6A5I3
-
-
Streptomyces viridosporus ATCC 14672 D6A5I3
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
Streptomyces viridosporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + O2 + H2O
-
Streptomyces viridosporus 2-oxoglutarate + NH3 + H2O2
-
?
L-glutamate + O2 + H2O
-
Streptomyces viridosporus ATCC 14672 2-oxoglutarate + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 69000, SDS-PAGE Streptomyces viridosporus

Synonyms

Synonyms Comment Organism
GLOD
-
Streptomyces viridosporus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Streptomyces viridosporus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 more than 60% activity between 20 and 50°C Streptomyces viridosporus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
approximately 90% of the initial enzyme activity remains after incubation at 40°C for 120 min, and about 70% activity remains after a 300 min pre-incubation at this temperature Streptomyces viridosporus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
Streptomyces viridosporus

pH Range

pH Minimum pH Maximum Comment Organism
5 7.5 more than 50% activity between pH 5.0 and 7.5 Streptomyces viridosporus

Cofactor

Cofactor Comment Organism Structure
FAD
-
Streptomyces viridosporus