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Literature summary for 1.4.3.11 extracted from

  • Niu, P.; Dong, X.; Wang, Y.; Liu, L.
    Enzymatic production of alpha-ketoglutaric acid from L-glutamic acid via L-glutamate oxidase (2014), J. Biotechnol., 179, 56-62 .
    View publication on PubMed
Search for more literature for 1.4.3.11

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptomyces viridosporus

Inhibitors

Inhibitors Comment Organism Structure
Ba2+ less than 60% residual activity at 3 mM Streptomyces viridosporus
Cu2+ less than 50% residual activity at 3 mM Streptomyces viridosporus
Fe2+ about 70% residual activity at 3 mM Streptomyces viridosporus
Zn2+ less than 40% residual activity at 3 mM Streptomyces viridosporus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 3 mM Mn2+ increases the enzyme activity towards L-glutamate by 19% Streptomyces viridosporus
additional information not influenced by Ca2+, Mg2+, Li+ and K+ Streptomyces viridosporus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + O2 + H2O Streptomyces viridosporus
-
 2-oxoglutarate + NH3 + H2O2
-
 ?
L-glutamate + O2 + H2O Streptomyces viridosporus ATCC14672
-
 2-oxoglutarate + NH3 + H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces viridosporus D6A5I3
-
-
Streptomyces viridosporus ATCC14672 D6A5I3
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Streptomyces viridosporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + O2 + H2O
-
 Streptomyces viridosporus 2-oxoglutarate + NH3 + H2O2
-
 ?
L-glutamate + O2 + H2O
-
 Streptomyces viridosporus ATCC14672 2-oxoglutarate + NH3 + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
LGOX
-
 Streptomyces viridosporus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Streptomyces viridosporus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10 40 the enzyme is stable at 10-30°C, and becomes instable when the temperature is higher than 40°C Streptomyces viridosporus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Streptomyces viridosporus

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Streptomyces viridosporus