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Literature summary for 1.4.3.11 extracted from

  • Liu, Q.; Ma, X.; Cheng, H.; Xu, N.; Liu, J.; Ma, Y.
    Co-expression of L-glutamate oxidase and catalase in Escherichia coli to produce alpha-ketoglutaric acid by whole-cell biocatalyst (2017), Biotechnol. Lett., 39, 913-919 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptomyces mobaraensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
9.3
-
L-glutamate at pH 6.0 and 35°C Streptomyces mobaraensis

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Na+, Li+, Mn2+, Mg2+ and Ca2+ at 5 mM have no detectable effect on the enzyme activity Streptomyces mobaraensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + O2 + H2O Streptomyces mobaraensis
-
2-oxoglutarate + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces mobaraensis A0A1S6XWY4
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni Sepharose column chromatography Streptomyces mobaraensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + O2 + H2O
-
Streptomyces mobaraensis 2-oxoglutarate + NH3 + H2O2
-
?
L-glutamine + O2 + H2O 3.6% activity compared to L-glutamate Streptomyces mobaraensis 2-oxoglutaramate + NH3 + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 64000, SDS-PAGE Streptomyces mobaraensis

Synonyms

Synonyms Comment Organism
LGOX
-
Streptomyces mobaraensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Streptomyces mobaraensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 50 more than 80% activity between 20 and 50°C Streptomyces mobaraensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 37 the enzyme remains stable between 20 and 37°C for 24 h Streptomyces mobaraensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Streptomyces mobaraensis

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 6 the recombinant enzyme retains about 90% of its initial activity after being incubated from pH 4.0 to 6.0 at 4°C for 24 h Streptomyces mobaraensis

Cofactor

Cofactor Comment Organism Structure
FAD
-
Streptomyces mobaraensis