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Literature summary for 1.4.3.11 extracted from

  • Wang, L.; Peng, R.; Tian, Y.; Liu, M.; Yao, Q.
    Isolation and characterization of a novel L-glutamate oxidase with strict substrate specificity from Streptomyces diastatochromogenes (2017), Biotechnol. Lett., 39, 523-528 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Streptomyces diastatochromogenes

General Stability

General Stability Organism
the 50% thermal inactivation temperature of the proteinase K treated enzyme is increased from 50 to 70°C Streptomyces diastatochromogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.15
-
L-glutamate proteinase K treated enzyme, at pH 8.0 and 30°C Streptomyces diastatochromogenes
0.66
-
L-glutamate recombinant enzyme, at pH 8.0 and 30°C Streptomyces diastatochromogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + O2 + H2O Streptomyces diastatochromogenes the enzyme exhibits strict specificity for L-glutamate 2-oxoglutarate + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces diastatochromogenes A0A1B1PF34
-
-

Purification (Commentary)

Purification (Comment) Organism
His trap Ni-NTA column chromatography Streptomyces diastatochromogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + O2 + H2O the enzyme exhibits strict specificity for L-glutamate Streptomyces diastatochromogenes 2-oxoglutarate + NH3 + H2O2
-
?
L-glutamine + O2 + H2O 0.63% activity compared to L-glutamate Streptomyces diastatochromogenes 2-oxoglutaramate + NH3 + H2O2
-
?

Synonyms

Synonyms Comment Organism
LGOX
-
Streptomyces diastatochromogenes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Streptomyces diastatochromogenes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
the 50% thermal inactivation temperature of recombinant enzyme is about 50°C Streptomyces diastatochromogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Streptomyces diastatochromogenes

Cofactor

Cofactor Comment Organism Structure
FAD
-
Streptomyces diastatochromogenes