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Literature summary for 1.4.3.11 extracted from

  • Utsumi, T.; Arima, J.; Sakaguchi, C.; Tamura, T.; Sasaki, C.; Kusakabe, H.; Sugio, S.; Inagaki, K.
    Arg305 of Streptomyces L-glutamate oxidase plays a crucial role for substrate recognition (2012), Biochem. Biophys. Res. Commun., 417, 951-955.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
mutant enzymes are expressed in Escherichia coli JM109 cells Streptomyces sp.

Protein Variants

Protein Variants Comment Organism
H312A the activity of the mutant enzyme toward L-glutamate is significantly reduced, however it exhibits catalytic activity toward various L-amino acids Streptomyces sp.
R305A the activity of the mutant enzyme toward L-glutamate is significantly reduced, however it exhibits catalytic activity toward various L-amino acids (best substrate is L-histidine) Streptomyces sp.
R305D the activity of the mutant enzyme toward L-glutamate is significantly reduced, however it exhibits catalytic activity toward various L-amino acids (best substrate is L-arginine) Streptomyces sp.
R305K the activity of the mutant enzyme toward L-glutamate is significantly reduced, however it exhibits catalytic activity toward various L-amino acids (best substrate is L-histidine) Streptomyces sp.
R305L the activity of the mutant enzyme toward L-glutamate is significantly reduced, however it exhibits catalytic activity toward various L-amino acids (best substrate is L-histidine) Streptomyces sp.
W564A the activity of the mutant enzyme toward L-glutamate is significantly reduced, however it exhibits catalytic activity toward various L-amino acids Streptomyces sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.173
-
L-glutamate wild type enzyme, pH and temperature not specified in the publication Streptomyces sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + O2 + H2O Streptomyces sp. specific substrate 2-oxoglutarate + NH3 + H2O2
-
?
additional information Streptomyces sp. no activity with L-Leu, L-Phe, L-Asn, L-His, and L-Arg ?
-
?

Organism

Organism UniProt Comment Textmining
Streptomyces sp. Q8L3C7
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE Toyopearl 650 column chromatography Streptomyces sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + O2 + H2O specific substrate Streptomyces sp. 2-oxoglutarate + NH3 + H2O2
-
?
additional information no activity with L-Leu, L-Phe, L-Asn, L-His, and L-Arg Streptomyces sp. ?
-
?

Synonyms

Synonyms Comment Organism
LGOX
-
Streptomyces sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
53.2
-
L-glutamate wild type enzyme, pH and temperature not specified in the publication Streptomyces sp.

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
308
-
L-glutamate wild type enzyme, pH and temperature not specified in the publication Streptomyces sp.