Application | Comment | Organism |
---|---|---|
analysis | the enzyme is useful as a component of amperometric L-glutamate sensors used in the food industry and clinical biochemistry | Streptomyces sp. |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain JM109 | Streptomyces sp. |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme, sitting drop vapor diffusion method, mixing of protein solution containing 10 mg/mL protein in 20 mM KPB, pH 7.4, with 5 m; dithiothreitol, with a reservoir solution containing 1.2 M NaH2PO4, 0.8 M K2HPO4, 200 mM LiSO4, 100 mM CAPS, pH 6.2, in a 1:2 ratio, 5°C, 3-4 weeks, X-ray diffraction structure determination and analysis at 2.6 A resolution | Streptomyces sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
10000 | - |
alpha2beta2gamma2, 2 * 40000-42000, alpha-subunit, 2 * 17000, beta-subunit, + 2 * 10000, gamma-subunit, SDS-PAGE and MALDI-TOF mass spectrometry | Streptomyces sp. |
17000 | - |
alpha2beta2gamma2, 2 * 40000-42000, alpha-subunit, 2 * 17000, beta-subunit, + 2 * 10000, gamma-subunit, SDS-PAGE and MALDI-TOF mass spectrometry | Streptomyces sp. |
75000 | - |
pre-mature enzyme, SDS-PAGE and MALDI-TOF mass spectrometry | Streptomyces sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + O2 + H2O | Streptomyces sp. | - |
2-oxoglutarate + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | Q8L3C7 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
proteolytic modification | LGOX is expressed as a single polypeptide precursor in an incompletely active form. It forms a mature enzyme with a hexameric alpha2beta2gamma2 structure formed through protease modification | Streptomyces sp. |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli strain JM109 | Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + O2 + H2O | - |
Streptomyces sp. | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
additional information | LGOX has a deeply buried active site and two entrances from the surface of the protein into the active site | Streptomyces sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | alpha2beta2gamma2, 2 * 40000-42000, alpha-subunit, 2 * 17000, beta-subunit, + 2 * 10000, gamma-subunit, SDS-PAGE and MALDI-TOF mass spectrometry | Streptomyces sp. |
monomer | 1 * 75000, pre-mature enzyme, SDS-PAGE and MALDI-TOF mass spectrometry | Streptomyces sp. |
More | the precursor of LGOX has a homodimeric structure and is less active than the mature enzyme with an alpha2beta2gamma2 structure, structure determination, overview. LGOX has a deeply buried active site and two entrances from the surface of the protein into the active site, amino acid sequence/primary structure analysis, overview | Streptomyces sp. |
Synonyms | Comment | Organism |
---|---|---|
LGOX | - |
Streptomyces sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the enzyme contains one FAD per trimer. The FAD prosthetic group is buried deeply within the enzyme, the isoalloxazine ring of FAD is positioned at the interface between the FAD-binding domain and the substrate-binding domain. It undergoes extensive interactions with the protein residues, overview | Streptomyces sp. |
General Information | Comment | Organism |
---|---|---|
additional information | the precursor of LGOX has a homodimeric structure and is less active than the mature enzyme with an alpha2beta2gamma2 structure, overview | Streptomyces sp. |