Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Paenarthrobacter aurescens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by spermidine and cadevarine | Paenarthrobacter aurescens | |
putrescine | substrate inhibition | Paenarthrobacter aurescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.3 | - |
putrescine | at pH 8.0 and 55°C | Paenarthrobacter aurescens | |
0.35 | - |
spermidine | at pH 8.0 and 55°C | Paenarthrobacter aurescens | |
0.36 | - |
cadaverine | at pH 8.0 and 55°C | Paenarthrobacter aurescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
putrescine + O2 + H2O | Paenarthrobacter aurescens | best substrate | 4-aminobutanal + NH3 + H2O2 | - |
? | |
putrescine + O2 + H2O | Paenarthrobacter aurescens TC1 | best substrate | 4-aminobutanal + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenarthrobacter aurescens | - |
- |
- |
Paenarthrobacter aurescens TC1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-Trap column chromatography | Paenarthrobacter aurescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cadaverine + O2 + H2O | 8.8% activity compared to putrescine | Paenarthrobacter aurescens | 5-aminopentanal + NH3 + H2O2 | - |
? | |
cadaverine + O2 + H2O | 8.8% activity compared to putrescine | Paenarthrobacter aurescens TC1 | 5-aminopentanal + NH3 + H2O2 | - |
? | |
additional information | no activity with histamine, tyramine, and 2-phenylethylamine | Paenarthrobacter aurescens | ? | - |
? | |
additional information | no activity with histamine, tyramine, and 2-phenylethylamine | Paenarthrobacter aurescens TC1 | ? | - |
? | |
putrescine + O2 + H2O | best substrate | Paenarthrobacter aurescens | 4-aminobutanal + NH3 + H2O2 | - |
? | |
putrescine + O2 + H2O | best substrate | Paenarthrobacter aurescens TC1 | 4-aminobutanal + NH3 + H2O2 | - |
? | |
spermidine + O2 + H2O | 2.5% activity compared to putrescine | Paenarthrobacter aurescens | 4-aminobutyraldehyde + 1,3-diaminopropane + H2O2 | - |
? | |
spermidine + O2 + H2O | 2.5% activity compared to putrescine | Paenarthrobacter aurescens TC1 | 4-aminobutyraldehyde + 1,3-diaminopropane + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
adenine dinucleotide-containing putrescine oxidase | - |
Paenarthrobacter aurescens |
APUO | - |
Paenarthrobacter aurescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Paenarthrobacter aurescens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.29 | - |
spermidine | at pH 8.0 and 55°C | Paenarthrobacter aurescens | |
1.72 | - |
cadaverine | at pH 8.0 and 55°C | Paenarthrobacter aurescens | |
31.8 | - |
putrescine | at pH 8.0 and 55°C | Paenarthrobacter aurescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Paenarthrobacter aurescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | the enzyme exhibits more than 90% of the maximum activity at pH 7.0 to 10.0 | Paenarthrobacter aurescens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
19.1 | - |
putrescine | at pH 8.0 and 55°C | Paenarthrobacter aurescens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.83 | - |
spermidine | at pH 8.0 and 55°C | Paenarthrobacter aurescens | |
4.78 | - |
cadaverine | at pH 8.0 and 55°C | Paenarthrobacter aurescens | |
106 | - |
putrescine | at pH 8.0 and 55°C | Paenarthrobacter aurescens |