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Literature summary for 1.4.3.10 extracted from

  • Lee, J.I.; Jang, J.H.; Yu, M.J.; Kim, Y.W.
    Construction of a bifunctional enzyme fusion for the combined determination of biogenic amines in foods (2013), J. Agric. Food Chem., 61, 9118-9124.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Paenarthrobacter aurescens

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibited by spermidine and cadevarine Paenarthrobacter aurescens
putrescine substrate inhibition Paenarthrobacter aurescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3
-
putrescine at pH 8.0 and 55°C Paenarthrobacter aurescens
0.35
-
spermidine at pH 8.0 and 55°C Paenarthrobacter aurescens
0.36
-
cadaverine at pH 8.0 and 55°C Paenarthrobacter aurescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
putrescine + O2 + H2O Paenarthrobacter aurescens best substrate 4-aminobutanal + NH3 + H2O2
-
?
putrescine + O2 + H2O Paenarthrobacter aurescens TC1 best substrate 4-aminobutanal + NH3 + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Paenarthrobacter aurescens
-
-
-
Paenarthrobacter aurescens TC1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His-Trap column chromatography Paenarthrobacter aurescens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cadaverine + O2 + H2O 8.8% activity compared to putrescine Paenarthrobacter aurescens 5-aminopentanal + NH3 + H2O2
-
?
cadaverine + O2 + H2O 8.8% activity compared to putrescine Paenarthrobacter aurescens TC1 5-aminopentanal + NH3 + H2O2
-
?
additional information no activity with histamine, tyramine, and 2-phenylethylamine Paenarthrobacter aurescens ?
-
?
additional information no activity with histamine, tyramine, and 2-phenylethylamine Paenarthrobacter aurescens TC1 ?
-
?
putrescine + O2 + H2O best substrate Paenarthrobacter aurescens 4-aminobutanal + NH3 + H2O2
-
?
putrescine + O2 + H2O best substrate Paenarthrobacter aurescens TC1 4-aminobutanal + NH3 + H2O2
-
?
spermidine + O2 + H2O 2.5% activity compared to putrescine Paenarthrobacter aurescens 4-aminobutyraldehyde + 1,3-diaminopropane + H2O2
-
?
spermidine + O2 + H2O 2.5% activity compared to putrescine Paenarthrobacter aurescens TC1 4-aminobutyraldehyde + 1,3-diaminopropane + H2O2
-
?

Synonyms

Synonyms Comment Organism
adenine dinucleotide-containing putrescine oxidase
-
Paenarthrobacter aurescens
APUO
-
Paenarthrobacter aurescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
Paenarthrobacter aurescens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.29
-
spermidine at pH 8.0 and 55°C Paenarthrobacter aurescens
1.72
-
cadaverine at pH 8.0 and 55°C Paenarthrobacter aurescens
31.8
-
putrescine at pH 8.0 and 55°C Paenarthrobacter aurescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Paenarthrobacter aurescens

pH Range

pH Minimum pH Maximum Comment Organism
7 10 the enzyme exhibits more than 90% of the maximum activity at pH 7.0 to 10.0 Paenarthrobacter aurescens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
19.1
-
putrescine at pH 8.0 and 55°C Paenarthrobacter aurescens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.83
-
spermidine at pH 8.0 and 55°C Paenarthrobacter aurescens
4.78
-
cadaverine at pH 8.0 and 55°C Paenarthrobacter aurescens
106
-
putrescine at pH 8.0 and 55°C Paenarthrobacter aurescens