Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Vanrija humicola |
Protein Variants | Comment | Organism |
---|---|---|
H56A | the mutant exhibits no significant activity toward acidic D-amino acids | Vanrija humicola |
H56A | the mutant gains the ability to utilize neutral D-amino acids as substrates, such as D-methionine, D-phenylalanine and D-glutamine | Vanrija humicola |
H56D | the mutant exhibits no significant activity toward acidic D-amino acids | Vanrija humicola |
H56F | the mutant exhibits no significant activity toward acidic D-amino acids | Vanrija humicola |
H56K | the mutant exhibits no significant activity toward acidic D-amino acids | Vanrija humicola |
H56N | the mutant exhibits no significant activity toward acidic D-amino acids | Vanrija humicola |
H56N | the mutant gains the ability to utilize neutral D-amino acids as substrates, such as D-methionine, D-phenylalanine and D-glutamine | Vanrija humicola |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-malate | 87.8% residual activity at 10 mM | Vanrija humicola | |
malonate | 62.1% residual activity at 10 mM | Vanrija humicola | |
additional information | not inhibited by 10 mM meso-tartrate, benzoate, anthranilate, and crotonate | Vanrija humicola |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
D-Asp | wild type enzyme, at pH 7.5 and 37°C | Vanrija humicola | |
8.5 | - |
D-Met | mutant enzyme H56A, at pH 7.5 and 37°C | Vanrija humicola | |
8.8 | - |
D-Leu | mutant enzyme H56N, at pH 7.5 and 37°C | Vanrija humicola | |
14.3 | - |
D-Phe | mutant enzyme H56A, at pH 7.5 and 37°C | Vanrija humicola | |
21 | - |
D-Gln | mutant enzyme H56N, at pH 7.5 and 37°C | Vanrija humicola | |
31.8 | - |
D-Met | mutant enzyme H56N, at pH 7.5 and 37°C | Vanrija humicola | |
34.8 | - |
D-Phe | mutant enzyme H56N, at pH 7.5 and 37°C | Vanrija humicola | |
39.4 | - |
D-Gln | mutant enzyme H56A, at pH 7.5 and 37°C | Vanrija humicola | |
83.4 | - |
D-Asn | mutant enzyme H56N, at pH 7.5 and 37°C | Vanrija humicola | |
106 | - |
D-Glu | wild type enzyme, at pH 7.5 and 37°C | Vanrija humicola |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Asp + H2O + O2 | Vanrija humicola | the enzyme shows high activity only with D-aspartate | oxaloacetate + NH3 + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vanrija humicola | Q75WF1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-Asn + H2O + O2 | low activity | Vanrija humicola | 2-oxosuccinamic acid + NH3 + H2O2 | - |
? | |
D-Asn + H2O + O2 | substrate for mutant enzyme H56N | Vanrija humicola | ? + NH3 + H2O2 | - |
? | |
D-Asp + H2O + O2 | the enzyme shows high activity only with D-aspartate | Vanrija humicola | oxaloacetate + NH3 + H2O2 | - |
? | |
D-Gln + H2O + O2 | substrate for mutant enzymes H56A and H56N | Vanrija humicola | ? + NH3 + H2O2 | - |
? | |
D-Glu + H2O + O2 | low activity | Vanrija humicola | 2-oxoglutarate + NH3 + H2O2 | - |
? | |
D-Leu + H2O + O2 | substrate for mutant enzyme H56N | Vanrija humicola | ? + NH3 + H2O2 | - |
? | |
D-Met + H2O + O2 | substrate for mutant enzymes H56A and H56N | Vanrija humicola | 4-(methylsulfonyl)-2-oxobutanoate + NH3 + H2O2 | - |
? | |
D-Phe + H2O + O2 | substrate for mutant enzymes H56A and H56N | Vanrija humicola | ? + NH3 + H2O2 | - |
? | |
additional information | no activity with D-Ala, D-Ser, D-Pro, D-Val, D-Thr, D-Ile, D-Leu, D-Gln, D-Met, D-Phe, D-Tyr, D-Trp, D-Lys, D-His, D-Arg, and Gly | Vanrija humicola | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Vanrija humicola |
Synonyms | Comment | Organism |
---|---|---|
DDO | - |
Vanrija humicola |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
D-Leu | mutant enzyme H56N, at pH 7.5 and 37°C | Vanrija humicola | |
2.2 | - |
D-Gln | mutant enzyme H56A, at pH 7.5 and 37°C | Vanrija humicola | |
2.6 | - |
D-Glu | wild type enzyme, at pH 7.5 and 37°C | Vanrija humicola | |
3.1 | - |
D-Phe | mutant enzyme H56A, at pH 7.5 and 37°C | Vanrija humicola | |
3.9 | - |
D-Asn | mutant enzyme H56N, at pH 7.5 and 37°C | Vanrija humicola | |
4 | - |
D-Met | mutant enzyme H56N, at pH 7.5 and 37°C | Vanrija humicola | |
4.1 | - |
D-Phe | mutant enzyme H56N, at pH 7.5 and 37°C | Vanrija humicola | |
4.5 | - |
D-Gln | mutant enzyme H56N, at pH 7.5 and 37°C | Vanrija humicola | |
11.5 | - |
D-Met | mutant enzyme H56A, at pH 7.5 and 37°C | Vanrija humicola | |
74.5 | - |
D-Asp | wild type enzyme, at pH 7.5 and 37°C | Vanrija humicola |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
D-Glu | wild type enzyme, at pH 7.5 and 37°C | Vanrija humicola | |
0.047 | - |
D-Asn | mutant enzyme H56N, at pH 7.5 and 37°C | Vanrija humicola | |
0.055 | - |
D-Gln | mutant enzyme H56A, at pH 7.5 and 37°C | Vanrija humicola | |
0.074 | - |
D-Leu | mutant enzyme H56N, at pH 7.5 and 37°C | Vanrija humicola | |
0.119 | - |
D-Phe | mutant enzyme H56N, at pH 7.5 and 37°C | Vanrija humicola | |
0.125 | - |
D-Met | mutant enzyme H56N, at pH 7.5 and 37°C | Vanrija humicola | |
0.213 | - |
D-Gln | mutant enzyme H56N, at pH 7.5 and 37°C | Vanrija humicola | |
0.218 | - |
D-Phe | mutant enzyme H56A, at pH 7.5 and 37°C | Vanrija humicola | |
1.35 | - |
D-Met | mutant enzyme H56A, at pH 7.5 and 37°C | Vanrija humicola | |
29.8 | - |
D-Asp | wild type enzyme, at pH 7.5 and 37°C | Vanrija humicola |