Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.4.3.1 extracted from

  • Katane, M.; Saitoh, Y.; Seida, Y.; Sekine, M.; Furuchi, T.; Homma, H.
    Comparative characterization of three D-aspartate oxidases and one D-amino acid oxidase from Caenorhabditis elegans (2010), Chem. Biodivers., 7, 1424-1434.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
genes F20Hp, C47Ap, and F18Ep, expression of DDO-1, DDO-2, and DDO-3 in Escherichia coli Caenorhabditis elegans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics in comparison to the human DDO, overview Caenorhabditis elegans
0.68
-
D-Glu pH 8.3, 37°C, recombinant DDO-3 Caenorhabditis elegans
0.8
-
D-Glu pH 8.3, 37°C, recombinant DDO-2 Caenorhabditis elegans
1.06
-
D-Glu pH 8.3, 37°C, recombinant DDO-1 Caenorhabditis elegans
3.81
-
D-Asp pH 8.3, 37°C, recombinant DDO-3 Caenorhabditis elegans
4.2
-
D-Asp pH 8.3, 37°C, recombinant DDO-2 Caenorhabditis elegans
5.54
-
D-Asp pH 8.3, 37°C, recombinant DDO-1 Caenorhabditis elegans
8.87
-
N-methyl-D-Asp pH 8.3, 37°C, recombinant DDO-3 Caenorhabditis elegans
9.23
-
N-methyl-D-Asp pH 8.3, 37°C, recombinant DDO-2 Caenorhabditis elegans
14.6
-
N-methyl-D-Asp pH 8.3, 37°C, recombinant DDO-1 Caenorhabditis elegans

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm DDO-2 Caenorhabditis elegans 5737
-
extracellular DDO-3 Caenorhabditis elegans
-
-
peroxisome DDO-1 Caenorhabditis elegans 5777
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37607
-
x * 37636, DDO-1, sequence calculation, x * 37607, DDO-2, sequence calculation, x * 42501, DDO-3, sequence calculation Caenorhabditis elegans
37636
-
x * 37636, DDO-1, sequence calculation, x * 37607, DDO-2, sequence calculation, x * 42501, DDO-3, sequence calculation Caenorhabditis elegans
42501
-
x * 37636, DDO-1, sequence calculation, x * 37607, DDO-2, sequence calculation, x * 42501, DDO-3, sequence calculation Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-Asp + H2O + O2 Caenorhabditis elegans
-
oxaloacetate + NH3 + H2O2
-
?
D-Glu + H2O + O2 Caenorhabditis elegans preferred substrate 2-oxoglutarate + NH3 + H2O2
-
?
N-methyl-D-Asp + H2O + O2 Caenorhabditis elegans
-
oxaloacetate + methylamine + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans
-
gene C47Ap encoding isozyme DDO-1, gene F18Ep encoding isozyme DDO-2, and gene F20Hp encoding isozyme DDO-3
-

Purification (Commentary)

Purification (Comment) Organism
recombinant DDO-1, DDO-2, and DDO-3 from Escherichia coli to homogeneity or near homogeneity Caenorhabditis elegans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Asp + H2O + O2
-
Caenorhabditis elegans oxaloacetate + NH3 + H2O2
-
?
D-Glu + H2O + O2 preferred substrate Caenorhabditis elegans 2-oxoglutarate + NH3 + H2O2
-
?
additional information all Caenorhabditis elegans DDOs exhibit the highest catalytic efficiency for D-Glu, but their efficiencies differ considerably for D-Glu, D-Asp, and NMDA. The isozymes show only very low or undetectable levels of activity against the neutral and basic D-amino acids and no activity with L-amino acids and N-methyl-L-Asp Caenorhabditis elegans ?
-
?
N-methyl-D-Asp + H2O + O2
-
Caenorhabditis elegans oxaloacetate + methylamine + H2O2
-
?

Subunits

Subunits Comment Organism
? x * 37636, DDO-1, sequence calculation, x * 37607, DDO-2, sequence calculation, x * 42501, DDO-3, sequence calculation Caenorhabditis elegans

Synonyms

Synonyms Comment Organism
DASPO
-
Caenorhabditis elegans
DDO
-
Caenorhabditis elegans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Caenorhabditis elegans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.76
-
D-Glu pH 8.3, 37°C, recombinant DDO-3 Caenorhabditis elegans
1.52
-
N-methyl-D-Asp pH 8.3, 37°C, recombinant DDO-3 Caenorhabditis elegans
2.51
-
D-Asp pH 8.3, 37°C, recombinant DDO-3 Caenorhabditis elegans
3.01
-
D-Glu pH 8.3, 37°C, recombinant DDO-2 Caenorhabditis elegans
6.04
-
N-methyl-D-Asp pH 8.3, 37°C, recombinant DDO-2 Caenorhabditis elegans
9.49
-
D-Asp pH 8.3, 37°C, recombinant DDO-2 Caenorhabditis elegans
26.1
-
D-Asp pH 8.3, 37°C, recombinant DDO-1 Caenorhabditis elegans
35.4
-
D-Glu pH 8.3, 37°C, recombinant DDO-1 Caenorhabditis elegans
84.5
-
N-methyl-D-Asp pH 8.3, 37°C, recombinant DDO-1 Caenorhabditis elegans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.3
-
assay at Caenorhabditis elegans

Cofactor

Cofactor Comment Organism Structure
FAD
-
Caenorhabditis elegans

General Information

General Information Comment Organism
physiological function D-Asp oxidase is a FAD-containing flavoprotein that catalyzes the oxidative deamination of D-amino acids with O2 to generate the corresponding 2-oxo acids, along with H2O2 and NH3. DDO is highly specific for acidic D-amino acids, such as D-Asp, N-methyl-D-Asp, and D-Glu Caenorhabditis elegans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
172
-
N-methyl-D-Asp pH 8.3, 37°C, recombinant DDO-3 Caenorhabditis elegans
654
-
N-methyl-D-Asp pH 8.3, 37°C, recombinant DDO-2 Caenorhabditis elegans
658
-
D-Asp pH 8.3, 37°C, recombinant DDO-3 Caenorhabditis elegans
1117
-
D-Glu pH 8.3, 37°C, recombinant DDO-3 Caenorhabditis elegans
1117
-
D-Glu pH 8.3, 37°C,recombinant DDO-3 Caenorhabditis elegans
2258
-
D-Asp pH 8.3, 37°C, recombinant DDO-2 Caenorhabditis elegans
3759
-
D-Glu pH 8.3, 37°C, recombinant DDO-2 Caenorhabditis elegans
3759
-
D-Glu pH 8.3, 37°C,recombinant DDO-2 Caenorhabditis elegans
4712
-
D-Asp pH 8.3, 37°C, recombinant DDO-1 Caenorhabditis elegans
5787
-
N-methyl-D-Asp pH 8.3, 37°C, recombinant DDO-1 Caenorhabditis elegans
33440
-
D-Glu pH 8.3, 37°C, recombinant DDO-1 Caenorhabditis elegans