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Literature summary for 1.4.1.B2 extracted from

  • Kreimeyer, A.; Perret, A.; Lechaplais, C.; Vallenet, D.; Medigue, C.; Salanoubat, M.; Weissenbach, J.
    Identification of the last unknown genes in the fermentation pathway of lysine (2007), J. Biol. Chem., 282, 7191-7197 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 DE3 cells unidentified

Inhibitors

Inhibitors Comment Organism Structure
NADPH the enzyme is 65% inhibited by the addition of 0.1 mM NADPH unidentified

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0593
-
NADP+ in the presence of 0.75 mM L-erythro-3,5-diaminohexanoate unidentified
0.1978
-
L-erythro-3,5-diaminohexanoate in the presence of 1 mM NADP+ unidentified
3.2
-
L-erythro-3,5-diaminohexanoate in the presence of 30 mM NAD+ unidentified
16.5
-
NAD+ in the presence of 15 mM L-erythro-3,5-diaminohexanoate unidentified

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39102
-
2 * 39102, calculated from sequence unidentified
40000
-
2 * 40000, SDS-PAGE unidentified

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-erythro-3,5-diaminohexanoate + H2O + NADP+ unidentified
-
(S)-5-amino-3-oxohexanoate + NH3 + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
unidentified A1X0G6 wastewater metagenomic collection
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, HisTrap FF column chromatography, MonoQ column chromatograhy, and Superdex 200 gel filtration unidentified

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-erythro-3,5-diaminohexanoate + H2O + NAD+
-
unidentified 5-amino-3-oxohexanoate + NH3 + NADH + H+
-
?
L-erythro-3,5-diaminohexanoate + H2O + NADP+
-
unidentified (S)-5-amino-3-oxohexanoate + NH3 + NADPH + H+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 40000, SDS-PAGE unidentified
dimer 2 * 39102, calculated from sequence unidentified

Synonyms

Synonyms Comment Organism
Kdd
-
unidentified

Cofactor

Cofactor Comment Organism Structure
NAD+ Vmax/Km for NADP+ is 223fold higher than Vmax/Km for NAD+ unidentified
NADP+ Vmax/Km for NADP+ is 223fold higher than Vmax/Km for NAD+ unidentified