Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 DE3 cells | unidentified |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADPH | the enzyme is 65% inhibited by the addition of 0.1 mM NADPH | unidentified |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0593 | - |
NADP+ | in the presence of 0.75 mM L-erythro-3,5-diaminohexanoate | unidentified | |
0.1978 | - |
L-erythro-3,5-diaminohexanoate | in the presence of 1 mM NADP+ | unidentified | |
3.2 | - |
L-erythro-3,5-diaminohexanoate | in the presence of 30 mM NAD+ | unidentified | |
16.5 | - |
NAD+ | in the presence of 15 mM L-erythro-3,5-diaminohexanoate | unidentified |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39102 | - |
2 * 39102, calculated from sequence | unidentified |
40000 | - |
2 * 40000, SDS-PAGE | unidentified |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-erythro-3,5-diaminohexanoate + H2O + NADP+ | unidentified | - |
(S)-5-amino-3-oxohexanoate + NH3 + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
unidentified | A1X0G6 | wastewater metagenomic collection | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, HisTrap FF column chromatography, MonoQ column chromatograhy, and Superdex 200 gel filtration | unidentified |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-erythro-3,5-diaminohexanoate + H2O + NAD+ | - |
unidentified | 5-amino-3-oxohexanoate + NH3 + NADH + H+ | - |
? | |
L-erythro-3,5-diaminohexanoate + H2O + NADP+ | - |
unidentified | (S)-5-amino-3-oxohexanoate + NH3 + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 40000, SDS-PAGE | unidentified |
dimer | 2 * 39102, calculated from sequence | unidentified |
Synonyms | Comment | Organism |
---|---|---|
Kdd | - |
unidentified |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | Vmax/Km for NADP+ is 223fold higher than Vmax/Km for NAD+ | unidentified | |
NADP+ | Vmax/Km for NADP+ is 223fold higher than Vmax/Km for NAD+ | unidentified |