KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
NAD+ | pH 10.5, 20°C | Sporosarcina psychrophila | |
0.65 | - |
L-leucine | pH 10.5, 20°C | Sporosarcina psychrophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
8 * 43000, SDS-PAGE | Sporosarcina psychrophila |
340000 | - |
gel filtration | Sporosarcina psychrophila |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-leucine + H2O + NAD+ | Sporosarcina psychrophila | - |
4-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
? | |
L-leucine + H2O + NAD+ | Sporosarcina psychrophila DSM 3 | - |
4-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sporosarcina psychrophila | - |
- |
- |
Sporosarcina psychrophila DSM 3 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 48fold by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography , and gel filtration to over 95% purity | Sporosarcina psychrophila |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.074 | - |
strain DSM 3, pH 10.5, 25°C | Sporosarcina psychrophila |
53.8 | - |
purified enzyme, pH 10.5, 25°C | Sporosarcina psychrophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-leucine + H2O + NAD+ | - |
Sporosarcina psychrophila | 4-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
? | |
L-leucine + H2O + NAD+ | - |
Sporosarcina psychrophila DSM 3 | 4-methyl-2-oxopentanoate + NH3 + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homooctamer | 8 * 43000, SDS-PAGE | Sporosarcina psychrophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Sporosarcina psychrophila |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
50 | highest activity at 50°C, 10% of maximal activity at 0°C | Sporosarcina psychrophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
purified enzyme, fully stable at pH 5.0-11.0 | Sporosarcina psychrophila |
40 | - |
purified enzyme, 40 min, fully stable | Sporosarcina psychrophila |
50 | - |
purified enzyme, half-life is 30 min | Sporosarcina psychrophila |
55 | - |
purified enzyme, half-life is 10 min | Sporosarcina psychrophila |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
L-leucine | pH 10.5, 20°C | Sporosarcina psychrophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
amination of 4-methyl-2-oxopentanoate | Sporosarcina psychrophila |
11 | - |
deamination of l-leucine | Sporosarcina psychrophila |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | purified enzyme, fully stable at 25°C | Sporosarcina psychrophila |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Sporosarcina psychrophila | |
NADH | - |
Sporosarcina psychrophila |