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Literature summary for 1.4.1.8 extracted from
- Purification and catalytic properties of L-valine dehydrogenase from Streptomyces cinnamonensis (1989), Biochem. J., 261, 853-861.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| L-valine | enzyme induced 100fold by L-valine | Streptomyces virginiae |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| (NH4)2SO4 | enzyme is sensitive to high concentrations | Streptomyces virginiae | |
| Ag+ | 0.01 mM in assay buffer | Streptomyces virginiae | |
| Ca2+ | 1 mM, partial inhibition | Streptomyces virginiae | |
| Cu2+ | 0.01 mM in assay buffer | Streptomyces virginiae | |
| Hg+ | - |
Streptomyces virginiae | |
| Hg2+ | - |
Streptomyces virginiae | |
| Mg2+ | 1 mM, partial inhibition | Streptomyces virginiae | |
| additional information | no inhibition by nucleotide mono-, di-, triphosphates or the free bases adenine, cytosine, thymine, guanine and uracil | Streptomyces virginiae | |
| p-chloromercuribenzoate | 0.01 mM in assay buffer | Streptomyces virginiae | |
| Urea | 3.5 M, complete and irreversible inactivation | Streptomyces virginiae | |
| Zn2+ | 1 mM, partial inhibition | Streptomyces virginiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.074 | - |
NADH | - |
Streptomyces virginiae | |
| 0.13 | - |
2-oxobutyrate | - |
Streptomyces virginiae | |
| 0.18 | - |
NAD+ | - |
Streptomyces virginiae | |
| 0.48 | - |
2-oxovalerate | - |
Streptomyces virginiae | |
| 0.5 | - |
2-oxoisocaproate | - |
Streptomyces virginiae | |
| 0.81 | - |
2-oxoisovalerate | - |
Streptomyces virginiae | |
| 0.81 | - |
2-oxocaproate | - |
Streptomyces virginiae | |
| 1.3 | - |
L-valine | - |
Streptomyces virginiae | |
| 3.2 | - |
L-norvaline | - |
Streptomyces virginiae | |
| 4 | - |
L-leucine | - |
Streptomyces virginiae | |
| 10.8 | - |
L-isoleucine | - |
Streptomyces virginiae | |
| 12.3 | - |
pyruvate | - |
Streptomyces virginiae | |
| 19.6 | - |
L-2-aminobutyrate | - |
Streptomyces virginiae | |
| 30 | - |
L-norleucine | - |
Streptomyces virginiae | |
| 55 | - |
NH3 | - |
Streptomyces virginiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41200 | - |
2 * 41200, SDS-PAGE | Streptomyces virginiae |
| 88000 | - |
gel filtration | Streptomyces virginiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-valine + NAD(P)+ + H2O | Streptomyces virginiae | first step in valine catabolism | 2-oxoisovalerate + NH3 + NAD(P)H | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces virginiae | - |
strains A 3823.5 and ATCC 15413 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 180fold purification | Streptomyces virginiae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| L-valine + H2O + NADP+ = 3-methyl-2-oxobutanoate + NH3 + NADPH + H+ | kinetic mechanism | Streptomyces virginiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 19.6 | - |
- |
Streptomyces virginiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, 25 mM sodium phosphate buffer pH 7, equal volume of glycerol, 3 months, stable | Streptomyces virginiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-2-aminobutyrate + H2O + NAD+ | L-2-aminobutyrate is identical with L-alpha-aminobutyrate | Streptomyces virginiae | 2-oxobutyrate + NADH + NH3 | 2-oxobutyrate is identical with alpha-ketobutyrate | r | |
| L-alanine + H2O + NAD+ | - |
Streptomyces virginiae | pyruvate + NH3 + NADH | pyruvate is identical with alpha-ketopropanoate and 2-oxopropanoate | ? | |
| L-isoleucine + H2O + NAD+ | - |
Streptomyces virginiae | 3-methyl-2-oxopentanoate + NADH + NH3 | - |
r | |
| L-leucine + H2O + NAD+ | - |
Streptomyces virginiae | 2-oxoisocaproate + NADH + NH3 | 2-oxoisocaproate is identical with 2-oxo-4-methylpentanoate, 2-oxoisohexanoate and alpha-ketoisocaproate | r | |
| L-norleucine + H2O + NAD+ | - |
Streptomyces virginiae | 2-oxocaproate + NADH + NH3 | 2-oxocaproate is identical with 2-oxohexanoate and alpha-keto-n-hexanoate | r | |
| L-norvaline + H2O + NAD+ | - |
Streptomyces virginiae | 2-oxovalerate + NH3 + NADH | optimal chain length for reductive amination | r | |
| L-norvaline + H2O + NAD+ | - |
Streptomyces virginiae | 2-oxovalerate + NH3 + NADH | 2-oxovalerate is identical with 2-oxopentanoate and alpha-ketovalerate | r | |
| L-valine + NAD(P)+ + H2O | NAD+ | Streptomyces virginiae | 2-oxoisovalerate + NH3 + NAD(P)H | NADH | r | |
| L-valine + NAD(P)+ + H2O | NAD+ | Streptomyces virginiae | 2-oxoisovalerate + NH3 + NAD(P)H | 2-oxoisovalerate is identical with 3-methyl-2-oxobutanoate and alpha-ketoisovalerate | r | |
| L-valine + NAD(P)+ + H2O | NAD+ | Streptomyces virginiae | 2-oxoisovalerate + NH3 + NAD(P)H | reductive amination: preferred substrate | r | |
| L-valine + NAD(P)+ + H2O | first step in valine catabolism | Streptomyces virginiae | 2-oxoisovalerate + NH3 + NAD(P)H | - |
? | |
| additional information | NH3 is the sole substrate as amino donor | Streptomyces virginiae | additional information | reductive amination: sequential ordered ternary-binary mechanism | ? | |
| additional information | pro-S hydrogen at C-4' of NADH is transferred to substrate, B-stereospecific | Streptomyces virginiae | additional information | reductive amination: sequential ordered ternary-binary mechanism | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 41200, SDS-PAGE | Streptomyces virginiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 21 | - |
assay at | Streptomyces virginiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.75 | - |
L-isoleucine | - |
Streptomyces virginiae | |
| 11.9 | - |
L-leucine | - |
Streptomyces virginiae | |
| 17.9 | - |
L-norleucine | - |
Streptomyces virginiae | |
| 18 | - |
L-norvaline | - |
Streptomyces virginiae | |
| 28.3 | - |
L-valine | - |
Streptomyces virginiae | |
| 71.9 | - |
2-oxoisocaproate | - |
Streptomyces virginiae | |
| 99 | - |
2-oxobutyrate | - |
Streptomyces virginiae | |
| 104 | - |
L-2-aminobutyrate | - |
Streptomyces virginiae | |
| 107 | - |
2-oxocaproate | - |
Streptomyces virginiae | |
| 114 | - |
pyruvate | - |
Streptomyces virginiae | |
| 164 | - |
2-oxovalerate | - |
Streptomyces virginiae | |
| 226 | - |
2-oxoisovalerate | - |
Streptomyces virginiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9.5 | - |
reductive amination | Streptomyces virginiae |
| 10 | - |
oxidative deamination | Streptomyces virginiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 10.5 | - |
above rates of reductive amination and oxidative deamination decrease dramatically | Streptomyces virginiae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
below markedly unstable | Streptomyces virginiae |
| 7.4 | - |
most stable | Streptomyces virginiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not with NADP+ and NADPH | Streptomyces virginiae | |
| NAD+ | NAD+ required, no use of NADP+ | Streptomyces virginiae | |
| NADH | NADH could not be replaced with NADPH for the reductive amination | Streptomyces virginiae | |
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