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Literature summary for 1.4.1.4 extracted from

  • Pathan, E.; Kulkarni, A.; Prasanna, N.; Ramana, C.; Deshpande, M.
    NADP-dependent glutamate dehydrogenases in a dimorphic zygomycete Benjaminiella poitrasii purification, characterization and their evaluation as an antifungal drug target (2020), Biochim. Biophys. Acta, 1864, 129696 .
    View publication on PubMed
Search for more literature for 1.4.1.4

Cloned(Commentary)

Cloned (Comment) Organism
gene gdh1, recombinant expression of His-tagged isozyme BpNADPGDH I in Escherichia coli strain BL21(DE3) Benjaminiella poitrasii
gene gdh3, recombinant expression of His-tagged isozyme BpNADPGDH II in Escherichia coli strain BL21(DE3) Benjaminiella poitrasii

Inhibitors

Inhibitors Comment Organism Structure
benzene-1,3-dicarboxamide
-
 Benjaminiella poitrasii
bis(2,2,2-trifluoroethyl) benzene-1,3-dicarboxylate
-
 Benjaminiella poitrasii
dibenzyl benzene-1,3-dicarboxylate
-
 Benjaminiella poitrasii
dimethyl 4,4'-[1,3-phenylenebis(carbonylazanediyl)]di(cyclopent-2-ene-1-carboxylate)
-
 Benjaminiella poitrasii
dimethyl benzene-1,3-dicarboxylate
-
 Benjaminiella poitrasii
dipropan-2-yl benzene-1,3-dicarboxylate
-
 Benjaminiella poitrasii
isophthalic acid
-
 Benjaminiella poitrasii
additional information structural analogues of L-glutamate, dimethyl esters of isophthalic acid (DMIP) and its derivatives are designed, synthesized and screened for inhibition of NADP-GDH activity as well as YeH transition in Benjaminiella poitrasii, and also in human pathogenic Candida albicans strains, effect of dimethyl esters of isophthalate (DMIP) derivatives on Benjaminiella poitrasii yeast-hypha transition in vivo, overview. The dimethyl esters of isophthalic acid (DMIP) compounds show a more pronounced effect on H-form specific BpNADPGDH II and inhibit YeH transition as well as growth in Benjaminiella poitrasii and Candida albicans strains; structural analogues of L-glutamate, dimethyl esters of isophthalic acid (DMIP) and its derivatives are designed, synthesized and screened for inhibition of NADP-GDH activity as well as YeH transition in Benjaminiella poitrasii, and also in human pathogenic Candida albicans strains, effect of dimethyl esters of isophthalate (DMIP) derivatives on Benjaminiella poitrasii yeast-hypha transition in vivo, overview. The dimethyl esters of isophthalic acid (DMIP) compounds show a more pronounced effect on H-form specific BpNADPGDH II and inhibit YeH transition as well as growth in Benjaminiella poitrasii and Candida albicans strains Benjaminiella poitrasii
N1,N1,N3,N3-tetra(propan-2-yl)benzene-1,3-dicarboxamide
-
 Benjaminiella poitrasii
N1,N3-bis(2-amino-2-oxoethyl)isophthalamide
-
 Benjaminiella poitrasii
N1,N3-bis(2-chlorophenyl)benzene-1,3-dicarboxamide
-
 Benjaminiella poitrasii
N1,N3-bis(3,5-dichlorophenyl)benzene-1,3-dicarboxamide
-
 Benjaminiella poitrasii
N1,N3-bis(3-fluorophenyl)benzene-1,3-dicarboxamide
-
 Benjaminiella poitrasii
N1,N3-bis(3-hydroxyphenyl)benzene-1,3-dicarboxamide
-
 Benjaminiella poitrasii
N1,N3-bis(4-fluorophenyl)benzene-1,3-dicarboxamide
-
 Benjaminiella poitrasii
N1,N3-bis(4-methoxyphenyl)benzene-1,3-dicarboxamide
-
 Benjaminiella poitrasii
N1,N3-bis(5-tert-butyl-2-hydroxyphenyl)benzene-1,3-dicarboxamide
-
 Benjaminiella poitrasii
N1,N3-bis[3,5-bis(trifluoromethyl)phenyl]benzene-1,3-dicarboxamide
-
 Benjaminiella poitrasii
N1,N3-bis[[3,5-bis(trifluoromethyl)phenyl]methyl]benzene-1,3-dicarboxamide
-
 Benjaminiella poitrasii
N1,N3-dihydroxybenzene-1,3-dicarboxamide
-
 Benjaminiella poitrasii
N1,N3-dimethoxybenzene-1,3-dicarboxamide
-
 Benjaminiella poitrasii
N1,N3-diphenylbenzene-1,3-dicarboxamide
-
 Benjaminiella poitrasii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
282000
-
 gel filtration Benjaminiella poitrasii
298000
-
 gel filtration Benjaminiella poitrasii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + H2O + NADP+ Benjaminiella poitrasii
-
 2-oxoglutarate + NH3 + NADPH + H+
-
 r
L-glutamate + H2O + NADP+ Benjaminiella poitrasii NCIM 1240
-
 2-oxoglutarate + NH3 + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Benjaminiella poitrasii A0A076N6W1
-
-
Benjaminiella poitrasii A0A076V390
-
-
Benjaminiella poitrasii NCIM 1240 A0A076N6W1
-
-
Benjaminiella poitrasii NCIM 1240 A0A076V390
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein cAMP-dependent phosphorylation, dephosphorylation, and rephosphorylation of purified NADP-GDHs and Ca2+-calmodulin (CaM) dependent phosphorylation of purified NADP-GDHs Benjaminiella poitrasii

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged isozyme BpNADPGDH I 36fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Benjaminiella poitrasii
recombinant His-tagged isozyme BpNADPGDH II 41fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Benjaminiella poitrasii

Source Tissue

Source Tissue Comment Organism Textmining
hypha
-
 Benjaminiella poitrasii
-
additional information hyphal-form specific BpNADPGDH II Benjaminiella poitrasii
-
additional information yeast-form specific isozyme BpNADPGDH I Benjaminiella poitrasii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NADP+
-
 Benjaminiella poitrasii 2-oxoglutarate + NH3 + NADPH + H+
-
 r
L-glutamate + H2O + NADP+
-
 Benjaminiella poitrasii NCIM 1240 2-oxoglutarate + NH3 + NADPH + H+
-
 r

Subunits

Subunits Comment Organism
homohexamer 6 * 47000, SDS-PAGE Benjaminiella poitrasii
homohexamer 6 * 49000, SDS-PAGE Benjaminiella poitrasii

Synonyms

Synonyms Comment Organism
BpNADPGDH I
-
 Benjaminiella poitrasii
BpNADPGDH II
-
 Benjaminiella poitrasii
GDH1
-
 Benjaminiella poitrasii
GDH3
-
 Benjaminiella poitrasii
hyphal-form specific BpNADPGDH II
-
 Benjaminiella poitrasii
NADP-dependent glutamate dehydrogenase
-
 Benjaminiella poitrasii
NADP-GDH
-
 Benjaminiella poitrasii
yeast-form specific BpNADPGDH I
-
 Benjaminiella poitrasii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 assay at room temperature Benjaminiella poitrasii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Benjaminiella poitrasii

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Benjaminiella poitrasii
NADPH
-
 Benjaminiella poitrasii

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information Ki values in microg/ml Benjaminiella poitrasii
0.0023
-
 N1,N3-bis[3,5-bis(trifluoromethyl)phenyl]benzene-1,3-dicarboxamide recombinant isozyme BpNADPGDH II, pH 8.0, 22°C Benjaminiella poitrasii
0.0034
-
 dimethyl benzene-1,3-dicarboxylate recombinant isozyme BpNADPGDH II, pH 8.0, 22°C Benjaminiella poitrasii
0.0041
-
 N1,N3-bis[3,5-bis(trifluoromethyl)phenyl]benzene-1,3-dicarboxamide recombinant isozyme BpNADPGDH I, pH 8.0, 22°C Benjaminiella poitrasii
0.0041
-
 N1,N3-bis(4-methoxyphenyl)benzene-1,3-dicarboxamide recombinant isozyme BpNADPGDH II, pH 8.0, 22°C Benjaminiella poitrasii
0.0054
-
 N1,N3-bis(4-methoxyphenyl)benzene-1,3-dicarboxamide recombinant isozyme BpNADPGDH I, pH 8.0, 22°C Benjaminiella poitrasii
0.0065
-
 dimethyl benzene-1,3-dicarboxylate recombinant isozyme BpNADPGDH I, pH 8.0, 22°C Benjaminiella poitrasii

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
additional information
-
 IC50 values in microg/ml, cytotoxic MIC90 and IC50 values, overview Benjaminiella poitrasii additional information
0.0015
-
 recombinant isozyme BpNADPGDH II, pH 8.0, 22°C Benjaminiella poitrasii bis(2,2,2-trifluoroethyl) benzene-1,3-dicarboxylate
0.0018
-
 recombinant isozyme BpNADPGDH II, pH 8.0, 22°C Benjaminiella poitrasii N1,N3-bis[[3,5-bis(trifluoromethyl)phenyl]methyl]benzene-1,3-dicarboxamide
0.0021
-
 recombinant isozyme BpNADPGDH II, pH 8.0, 22°C Benjaminiella poitrasii N1,N3-bis(4-fluorophenyl)benzene-1,3-dicarboxamide
0.0023
-
 recombinant isozyme BpNADPGDH I, pH 8.0, 22°C Benjaminiella poitrasii bis(2,2,2-trifluoroethyl) benzene-1,3-dicarboxylate
0.0026
-
 recombinant isozyme BpNADPGDH I, pH 8.0, 22°C Benjaminiella poitrasii N1,N3-bis(4-fluorophenyl)benzene-1,3-dicarboxamide
0.0029
-
 recombinant isozyme BpNADPGDH I, pH 8.0, 22°C Benjaminiella poitrasii N1,N3-bis[[3,5-bis(trifluoromethyl)phenyl]methyl]benzene-1,3-dicarboxamide
0.0032
-
 recombinant isozyme BpNADPGDH I, pH 8.0, 22°C Benjaminiella poitrasii N1,N3-bis(2-chlorophenyl)benzene-1,3-dicarboxamide
0.0035
-
 recombinant isozyme BpNADPGDH I, pH 8.0, 22°C Benjaminiella poitrasii dimethyl 4,4'-[1,3-phenylenebis(carbonylazanediyl)]di(cyclopent-2-ene-1-carboxylate)
0.0035
-
 recombinant isozyme BpNADPGDH I, pH 8.0, 22°C Benjaminiella poitrasii N1,N3-bis(3-fluorophenyl)benzene-1,3-dicarboxamide
0.0035
-
 recombinant isozyme BpNADPGDH II, pH 8.0, 22°C Benjaminiella poitrasii N1,N3-bis(2-chlorophenyl)benzene-1,3-dicarboxamide
0.0037
-
 recombinant isozyme BpNADPGDH II, pH 8.0, 22°C Benjaminiella poitrasii N1,N3-dimethoxybenzene-1,3-dicarboxamide
0.0038
-
 recombinant isozyme BpNADPGDH I, pH 8.0, 22°C Benjaminiella poitrasii N1,N3-dimethoxybenzene-1,3-dicarboxamide
0.0039
-
 recombinant isozyme BpNADPGDH II, pH 8.0, 22°C Benjaminiella poitrasii N1,N3-bis(3-fluorophenyl)benzene-1,3-dicarboxamide
0.0043
-
 recombinant isozyme BpNADPGDH I, pH 8.0, 22°C Benjaminiella poitrasii N1,N3-bis(3,5-dichlorophenyl)benzene-1,3-dicarboxamide
0.0043
-
 recombinant isozyme BpNADPGDH II, pH 8.0, 22°C Benjaminiella poitrasii dimethyl 4,4'-[1,3-phenylenebis(carbonylazanediyl)]di(cyclopent-2-ene-1-carboxylate)
0.0049
-
 recombinant isozyme BpNADPGDH I, pH 8.0, 22°C Benjaminiella poitrasii N1,N3-dihydroxybenzene-1,3-dicarboxamide
0.0051
-
 recombinant isozyme BpNADPGDH II, pH 8.0, 22°C Benjaminiella poitrasii N1,N3-dihydroxybenzene-1,3-dicarboxamide
0.0053
-
 recombinant isozyme BpNADPGDH I, pH 8.0, 22°C Benjaminiella poitrasii N1,N3-bis(2-amino-2-oxoethyl)isophthalamide
0.0058
-
 recombinant isozyme BpNADPGDH II, pH 8.0, 22°C Benjaminiella poitrasii N1,N3-bis(2-amino-2-oxoethyl)isophthalamide
0.0059
-
 recombinant isozyme BpNADPGDH II, pH 8.0, 22°C Benjaminiella poitrasii N1,N3-bis[3,5-bis(trifluoromethyl)phenyl]benzene-1,3-dicarboxamide
0.0061
-
 recombinant isozyme BpNADPGDH II, pH 8.0, 22°C Benjaminiella poitrasii N1,N3-bis(3,5-dichlorophenyl)benzene-1,3-dicarboxamide
0.0062
-
 recombinant isozyme BpNADPGDH I, pH 8.0, 22°C Benjaminiella poitrasii N1,N3-bis(5-tert-butyl-2-hydroxyphenyl)benzene-1,3-dicarboxamide
0.0062
-
 recombinant isozyme BpNADPGDH I, pH 8.0, 22°C Benjaminiella poitrasii N1,N3-bis[3,5-bis(trifluoromethyl)phenyl]benzene-1,3-dicarboxamide
0.0068
-
 recombinant isozyme BpNADPGDH II, pH 8.0, 22°C Benjaminiella poitrasii N1,N3-bis(4-methoxyphenyl)benzene-1,3-dicarboxamide
0.007
-
 recombinant isozyme BpNADPGDH I, pH 8.0, 22°C Benjaminiella poitrasii N1,N3-bis(4-methoxyphenyl)benzene-1,3-dicarboxamide
0.0073
-
 recombinant isozyme BpNADPGDH I, pH 8.0, 22°C Benjaminiella poitrasii N1,N3-bis(3-hydroxyphenyl)benzene-1,3-dicarboxamide
0.0073
-
 recombinant isozyme BpNADPGDH II, pH 8.0, 22°C Benjaminiella poitrasii N1,N3-bis(5-tert-butyl-2-hydroxyphenyl)benzene-1,3-dicarboxamide
0.0081
-
 recombinant isozyme BpNADPGDH II, pH 8.0, 22°C Benjaminiella poitrasii N1,N3-bis(3-hydroxyphenyl)benzene-1,3-dicarboxamide
0.0122
-
 recombinant isozyme BpNADPGDH I, pH 8.0, 22°C Benjaminiella poitrasii dimethyl benzene-1,3-dicarboxylate
0.0125
-
 recombinant isozyme BpNADPGDH II, pH 8.0, 22°C Benjaminiella poitrasii dimethyl benzene-1,3-dicarboxylate

General Information

General Information Comment Organism
physiological function besides the distinct kinetic properties, the two isozymes in Benjaminiella poitrasii, BpNADPGDH I and BpNADPGDH II, are regulated by cAMP-dependent- and calmodulin (CaM) dependent protein kinases, respectively Benjaminiella poitrasii
physiological function besides the distinct kinetic properties, the two isozymes in Benjaminiella poitrasii, BpNADPGDH I and BpNADPGDH II, are regulated by cAMP-dependent- and calmodulin (CaM)-dependent protein kinases, respectively Benjaminiella poitrasii