Inhibitors | Comment | Organism | Structure |
---|---|---|---|
isophthalate | a competitive inhibitor of glutamate dehydrogenase, is involved in C and N metabolism | Acinetobacter lwoffii | |
isophthalate | a competitive inhibitor of glutamate dehydrogenase, is involved in C and N metabolism | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic analysis, overview, the isozyme GDHI' shows negative cooperativity with NH4+ substrate, with a decreased affinity for NH4Cl | Acinetobacter lwoffii | |
additional information | - |
additional information | kinetic analysis, overview, the isozymes show negative cooperativity with NH4+ substrate, with a decreased affinity for NH4Cl | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
4 * 43000, isozyme GDHI, SDS-PAGE | Acinetobacter lwoffii |
43000 | - |
4 * 43000, isozymes GDHI and GDHII, SDS-PAGE | Pseudomonas aeruginosa |
170000 | - |
isozymes GDHI and GDHII, gel filtration | Pseudomonas aeruginosa |
250000 | - |
isozyme GDHI', gel filtration | Acinetobacter lwoffii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NADP+ | Pseudomonas aeruginosa | - |
2-oxoglutarate + NH3 + NADPH + H+ | - |
r | |
L-glutamate + H2O + NADP+ | Acinetobacter lwoffii | - |
2-oxoglutarate + NH3 + NADPH + H+ | - |
r | |
L-glutamate + H2O + NADP+ | Pseudomonas aeruginosa PP4 | - |
2-oxoglutarate + NH3 + NADPH + H+ | - |
r | |
L-glutamate + H2O + NADP+ | Acinetobacter lwoffii ISP4 | - |
2-oxoglutarate + NH3 + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter lwoffii | - |
NADPH-GDH isozymes GDHI, GDHII, and GDHI' | - |
Acinetobacter lwoffii ISP4 | - |
NADPH-GDH isozymes GDHI, GDHII, and GDHI' | - |
Pseudomonas aeruginosa | - |
NADPH-GDH isozymes GDHI, GDHII, and GDHI' | - |
Pseudomonas aeruginosa PP4 | - |
NADPH-GDH isozymes GDHI, GDHII, and GDHI' | - |
Purification (Comment) | Organism |
---|---|
native GDHI' 142fold from cells grown on isophthalate and 510fold from cells grown on 2YT | Acinetobacter lwoffii |
native isozymes GDHI and GDHII from strain PP4 grown on isophthalate and glucose, respectively. Enzyme yields are better in the presence of 1 mM 2-oxoglutarate, 1 mM EDTA, and 4.35% glycerol. GDHI and GDHII are purified 228fold and 428fold, respectively | Pseudomonas aeruginosa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:isophthalate-grown cell | isophthalate as a sole source of carbon and energy. Strain ISP4 produces a single form of NADP-GDH, GDHI' when it is grown on either isophthalate or rich medium 2YT | Acinetobacter lwoffii | - |
culture condition:isophthalate-grown cell | isophthalate as a sole source of carbon and energy. Strain PP4 shows carbon source-dependent modulation of NADP-GDH. GDHI is produced when cells are grown on isophthalate | Pseudomonas aeruginosa | - |
Storage Stability | Organism |
---|---|
4°C, purified enzyme, stable for about 5 to 6 months without any significant loss of activity | Acinetobacter lwoffii |
4°C, purified enzymes, stable for about 5 to 6 months without any significant loss of activity | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NADP+ | - |
Pseudomonas aeruginosa | 2-oxoglutarate + NH3 + NADPH + H+ | - |
r | |
L-glutamate + H2O + NADP+ | - |
Acinetobacter lwoffii | 2-oxoglutarate + NH3 + NADPH + H+ | - |
r | |
L-glutamate + H2O + NADP+ | - |
Pseudomonas aeruginosa | 2-oxoglutarate + NH3 + NADPH + H+ | usage of NH4Cl as substrate for the reverse reaction | r | |
L-glutamate + H2O + NADP+ | usage of NH4Cl as substrate for the reverse reaction | Acinetobacter lwoffii | 2-oxoglutarate + NH3 + NADPH + H+ | - |
r | |
L-glutamate + H2O + NADP+ | - |
Pseudomonas aeruginosa PP4 | 2-oxoglutarate + NH3 + NADPH + H+ | - |
r | |
L-glutamate + H2O + NADP+ | - |
Pseudomonas aeruginosa PP4 | 2-oxoglutarate + NH3 + NADPH + H+ | usage of NH4Cl as substrate for the reverse reaction | r | |
L-glutamate + H2O + NADP+ | - |
Acinetobacter lwoffii ISP4 | 2-oxoglutarate + NH3 + NADPH + H+ | - |
r | |
L-glutamate + H2O + NADP+ | usage of NH4Cl as substrate for the reverse reaction | Acinetobacter lwoffii ISP4 | 2-oxoglutarate + NH3 + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homohexamer | 4 * 43000, isozyme GDHI', SDS-PAGE | Acinetobacter lwoffii |
homotetramer | 4 * 43000, isozymes GDHI and GDHII, SDS-PAGE | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
GDHI | - |
Pseudomonas aeruginosa |
GDHI' | - |
Acinetobacter lwoffii |
NADP-GDH | - |
Pseudomonas aeruginosa |
NADP-GDH | - |
Acinetobacter lwoffii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | reverse reaction, assay at | Pseudomonas aeruginosa |
8 | 8.5 | reverse reaction, assay at | Acinetobacter lwoffii |
9.5 | - |
forward reaction, assay at | Pseudomonas aeruginosa |
9.5 | - |
forward reaction, assay at | Acinetobacter lwoffii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Pseudomonas aeruginosa | |
NADP+ | - |
Acinetobacter lwoffii | |
NADPH | - |
Pseudomonas aeruginosa | |
NADPH | - |
Acinetobacter lwoffii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Ki values for isophthalate with different isozymes from differently grown strains in forward and reverse reactions, overview | Pseudomonas aeruginosa | |
additional information | - |
additional information | Ki values for isophthalate with isozyme from differently grown strains in forward and reverse reactions, overview | Acinetobacter lwoffii |