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Literature summary for 1.4.1.4 extracted from

  • Epstein, I.; Grossowicz, N.
    Purification and properties of glutamate dehydrogenase from a thermophilic bacillus (1975), J. Bacteriol., 122, 1257-1264.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.053
-
NADPH reductive amination Thermophilic bacillus
0.3
-
NADP+ oxidative deamination Thermophilic bacillus
1.1
-
L-glutamate oxidative deamination Thermophilic bacillus
1.3
-
2-oxoglutarate reductive amination Thermophilic bacillus
21
-
NH4+ reductive amination Thermophilic bacillus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + NADP+ + H2O Thermophilic bacillus
-
2-oxoglutarate + NADPH + NH3
-
r

Organism

Organism UniProt Comment Textmining
Thermophilic bacillus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermophilic bacillus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
46.2
-
reductive amination at 55°C Thermophilic bacillus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH4+ + NADPH
-
Thermophilic bacillus L-glutamate + NADP+
-
?
L-glutamate + NADP+ + H2O
-
Thermophilic bacillus 2-oxoglutarate + NADPH + NH3
-
r

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
after 300 min 60% activity Thermophilic bacillus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
reductive amination Thermophilic bacillus
8.4
-
oxidative deamination Thermophilic bacillus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.7
-
inactivation Thermophilic bacillus
5.8 9 highly thermostable Thermophilic bacillus

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Thermophilic bacillus
NADPH
-
Thermophilic bacillus