General Stability | Organism |
---|---|
inactivated by freezing/thawing | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
guanidine hydrochloride | complete loss of activity | Escherichia coli | |
L-homoserine | competitive inhibitor with respect to both ammonia and glutamine | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
NADPH | reductive amination | Escherichia coli | |
0.042 | - |
NADP+ | oxidative deamination | Escherichia coli | |
0.64 | - |
2-oxoglutarate | reductive amination | Escherichia coli | |
1.1 | - |
NH4+ | reductive amination | Escherichia coli | |
1.3 | - |
L-glutamate | oxidative deamination | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
alpha6, 6 * 50000, SDS-PAGE | Escherichia coli |
300000 | - |
sedimentation equilibrium | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | Escherichia coli | - |
2-oxoglutarate + NADPH + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
180 | - |
oxidative deamination at 25°C | Escherichia coli |
Storage Stability | Organism |
---|---|
4°C, water, 20 mM potassium phosphate or sodium phosphate, pH 7.2, at least 3 months | Escherichia coli |
room temperature, several months stable | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH4+ + NADPH | - |
Escherichia coli | L-glutamate + NADP+ | - |
? | |
L-glutamate + NADP+ + H2O | - |
Escherichia coli | 2-oxoglutarate + NADPH + NH3 | - |
r |
Subunits | Comment | Organism |
---|---|---|
homohexamer | alpha6, 6 * 50000, SDS-PAGE | Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
inactivated by freezing | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Escherichia coli | |
NADPH | - |
Escherichia coli |