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Literature summary for 1.4.1.4 extracted from
- Glutamate dehydrogenase from Escherichia coli: induction, purification and properties of the enzyme (1975), Biochim. Biophys. Acta, 377, 217-228.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| AMP | slight activation up to 1 mM | Escherichia coli |  |
| ATP | at 2 mM, 50% activation | Escherichia coli | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | inhibitory at higher concentrations than 1 mM | Escherichia coli | |
| Urea | at 8 M stable for 10 min | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
x * 46000, SDS-PAGE | Escherichia coli |
| 245000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + NADP+ + H2O | Escherichia coli | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
| L-glutamate + NADP+ + H2O | Escherichia coli MRE 600 | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli MRE 600 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 250 | - |
oxidative deamination | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 0.1 M phosphate buffer, several days, no loss of activity | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + NADP+ + H2O | - |
Escherichia coli | 2-oxoglutarate + NADPH + NH3 | - |
r | |
| L-glutamate + NADP+ + H2O | - |
Escherichia coli MRE 600 | 2-oxoglutarate + NADPH + NH3 | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 46000, SDS-PAGE | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
reductive amination | Escherichia coli |
| 9 | - |
oxidative deamination | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Escherichia coli | |
| NADPH | - |
Escherichia coli | |
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