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Literature summary for 1.4.1.4 extracted from
- Purification and properties of NADP-dependent glutamate dehydrogenase from yeast nuclear fractions (1976), Biochim. Biophys. Acta, 429, 324-330.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| potassium phosphate | up to 0.1 M oxidative deamination | Saccharomyces cerevisiae |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| potassium phosphate | over 0.1 M at oxidative deamination | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.028 | - |
NADPH | reductive amination | Saccharomyces cerevisiae | |
| 0.12 | - |
NADP+ | oxidative deamination | Saccharomyces cerevisiae | |
| 0.4 | - |
2-oxoglutarate | reductive amination | Saccharomyces cerevisiae | |
| 10 | - |
NH4+ | reductive amination | Saccharomyces cerevisiae | |
| 10 | - |
L-glutamate | oxidative deamination | Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Saccharomyces cerevisiae | 5634 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate + NADP+ + H2O | Saccharomyces cerevisiae | - |
2-oxoglutarate + NADPH + NH3 | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2.54 | - |
reductive amination | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-oxoglutarate + NH4+ + NADPH | - |
Saccharomyces cerevisiae | L-glutamate + NADP+ | - |
? | |
| L-glutamate + NADP+ + H2O | - |
Saccharomyces cerevisiae | 2-oxoglutarate + NADPH + NH3 | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.7 | - |
reductive amination | Saccharomyces cerevisiae |
| 9 | - |
oxidative deamination | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Saccharomyces cerevisiae | |
| NADPH | - |
Saccharomyces cerevisiae | |
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