General Stability | Organism |
---|---|
in absence of salt it is irreversibly inactivated, optimal NaCl concentration is 1.1 M | Halobacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
NADPH | reductive amination | Halobacterium sp. | |
0.061 | - |
NADP+ | oxidative deamination | Halobacterium sp. | |
3 | - |
2-oxoglutarate | reductive amination at 25°C | Halobacterium sp. | |
14.2 | - |
L-glutamate | oxidative deamination | Halobacterium sp. | |
16.6 | - |
NH4+ | reductive amination | Halobacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53500 | - |
alpha4, 4 * 53500, SDS-PAGE | Halobacterium sp. |
212000 | - |
sedimentation equilibrium and sedimentation velocity and diffusion coefficient | Halobacterium sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | Halobacterium sp. | - |
2-oxoglutarate + NADPH + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Halobacterium sp. | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH4+ + NADPH | - |
Halobacterium sp. | L-glutamate + NADP+ | - |
? | |
L-glutamate + NADP+ + H2O | - |
Halobacterium sp. | 2-oxoglutarate + NADPH + NH3 | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | alpha4, 4 * 53500, SDS-PAGE | Halobacterium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
at 4.3 M NaCl stable for several h | Halobacterium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Halobacterium sp. | |
NADPH | - |
Halobacterium sp. |