Activating Compound | Comment | Organism | Structure |
---|---|---|---|
GDP | at 1 mM five-fold Michaelis Menten constant of 2-oxoglutarate | Trichomonas vaginalis | |
IDP | at 1 mM five-fold Michaelis Menten constant of 2-oxoglutarate | Trichomonas vaginalis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | competitive inhibitor of the deamination | Trichomonas vaginalis | |
glutamate | competitive inhibitor of the amination reaction | Trichomonas vaginalis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADPH | reductive amination | Trichomonas vaginalis | |
0.04 | - |
NADP+ | oxidative deamination | Trichomonas vaginalis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Trichomonas vaginalis | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
230000 | - |
gel filtration | Trichomonas vaginalis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | Trichomonas vaginalis | - |
2-oxoglutarate + NADPH + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trichomonas vaginalis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partially | Trichomonas vaginalis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
133 | - |
reductive amination at 37°C and pH 6.7 | Trichomonas vaginalis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | - |
Trichomonas vaginalis | 2-oxoglutarate + NADPH + NH3 | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
57 | - |
after 1 h stable | Trichomonas vaginalis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.7 | - |
reductive amination | Trichomonas vaginalis |
8 | - |
oxidative deamination | Trichomonas vaginalis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Trichomonas vaginalis | |
NADPH | - |
Trichomonas vaginalis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
2-oxoglutarate | competitive | Trichomonas vaginalis | |
5.6 | - |
glutamate | competitive inhibitor | Trichomonas vaginalis |