General Stability | Organism |
---|---|
unstable when frozen at -20°C after a few h even with addition of 50% v/v glycerol | Phormidium laminosum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Glutaric acid | at 20 mM, 25% inhibition | Phormidium laminosum | |
L-Glutamic acid | at 20 mM 25% inhibition | Phormidium laminosum | |
L-tryptophan | at 20 mM, 15% inhibition | Phormidium laminosum | |
Urea | no activity at 6 mM | Phormidium laminosum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.064 | - |
NADPH | reductive amination | Phormidium laminosum | |
1.25 | - |
2-oxoglutarate | reductive amination | Phormidium laminosum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
280000 | - |
gel filtration | Phormidium laminosum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | Phormidium laminosum | - |
2-oxoglutarate + NADPH + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phormidium laminosum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Phormidium laminosum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
32.9 | - |
reductive amination at 30°C | Phormidium laminosum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | - |
Phormidium laminosum | 2-oxoglutarate + NADPH + NH3 | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
reductive amination | Phormidium laminosum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
72 | - |
complete inactivation | Phormidium laminosum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
reductive amination in potassium phosphate buffer | Phormidium laminosum |
8 | - |
reductive amination in Tris hydrochloride buffer | Phormidium laminosum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Phormidium laminosum | |
NADPH | - |
Phormidium laminosum |