Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | at 1 mM 27% inhibition | Escherichia coli | |
Zn2+ | at 1 mM, 40% inhibition | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.083 | - |
NADPH | reductive amination | Escherichia coli | |
0.11 | - |
NADP+ | oxidative deamination | Escherichia coli | |
2.2 | - |
L-glutamate | oxidative deamination | Escherichia coli | |
3.25 | - |
2-oxoglutarate | reductive amination | Escherichia coli | |
36 | - |
NH4+ | reductive amination | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44500 | - |
alpha6, 6 * 44500, SDS-PAGE | Escherichia coli |
275000 | - |
gel filtration | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | Escherichia coli | - |
2-oxoglutarate + NADPH + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH4+ + NADPH | - |
Escherichia coli | L-glutamate + NADP+ | - |
? | |
L-glutamate + NADP+ + H2O | - |
Escherichia coli | 2-oxoglutarate + NADPH + NH3 | - |
r |
Subunits | Comment | Organism |
---|---|---|
homohexamer | alpha6, 6 * 44500, SDS-PAGE | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Escherichia coli | |
NADPH | - |
Escherichia coli |