Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Hg2+ | at 1 mM, 100% activity loss | Bryopsis maxima | |
Mg2+ | at 1 mM, 16% activity loss | Bryopsis maxima | |
Mn2+ | at 1 mM, 19% activity loss | Bryopsis maxima | |
Zn2+ | at 1 mM, 64% activity loss | Bryopsis maxima |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADP+ | oxidative deamination at 25°C | Bryopsis maxima | |
0.03 | - |
NADPH | reductive amination at 25°C | Bryopsis maxima | |
2.2 | - |
NH4+ | reductive amination at 25°C | Bryopsis maxima | |
3 | - |
2-oxoglutarate | reductive amination at 25°C | Bryopsis maxima | |
3.2 | - |
L-glutamate | oxidative deamination at 25°C | Bryopsis maxima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
alpha6, 6 * 46000, SDS-PAGE | Bryopsis maxima |
280000 | - |
gel filtration | Bryopsis maxima |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + NADP+ + H2O | Bryopsis maxima | - |
2-oxoglutarate + NADPH + NH3 | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bryopsis maxima | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bryopsis maxima |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18.4 | - |
oxidative deamination at 25°C | Bryopsis maxima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NH4+ + NADPH | - |
Bryopsis maxima | L-glutamate + NADP+ | - |
? | |
L-glutamate + NADP+ + H2O | - |
Bryopsis maxima | 2-oxoglutarate + NADPH + NH3 | - |
r |
Subunits | Comment | Organism |
---|---|---|
homohexamer | alpha6, 6 * 46000, SDS-PAGE | Bryopsis maxima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
maximal temperature for activity, 60 min stable | Bryopsis maxima |
50 | - |
10 min stable | Bryopsis maxima |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
reductive amination | Bryopsis maxima |
8.6 | - |
oxidative deamination | Bryopsis maxima |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.2 | 8 | - |
Bryopsis maxima |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Bryopsis maxima | |
NADPH | - |
Bryopsis maxima |