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Literature summary for 1.4.1.4 extracted from

  • Helianti, I.; Morita, Y.; Yamamura, A.; Murakami, Y.; Yokoyama, K.; Tamiya, E.
    Characterization of native glutamate dehydrogenase from an aerobic hyperthermophilic archaeon Aeropyrum pernix K1 (2001), Appl. Microbiol. Biotechnol., 56, 388-394.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
acetonitrile activates Aeropyrum pernix K1
ethanol activates up to 40% v/v Aeropyrum pernix K1
methanol activates Aeropyrum pernix K1

General Stability

General Stability Organism
1 mg/ml bovine serum albumin stabilizes Aeropyrum pernix K1

Inhibitors

Inhibitors Comment Organism Structure
guanidine hydrochloride complete loss of activity Aeropyrum pernix K1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.049
-
NADPH reductive amination Aeropyrum pernix K1
0.053
-
NADP+ oxidative deamination Aeropyrum pernix K1
0.32
-
2-oxoglutarate reductive amination Aeropyrum pernix K1
4.56
-
NH3 reductive amination Aeropyrum pernix K1
5.18
-
L-glutamate oxidative deamination Aeropyrum pernix K1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
alpha6, 6 * 47000, SDS-PAGE Aeropyrum pernix K1
285000
-
gel filtration Aeropyrum pernix K1

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + NADP+ + H2O Aeropyrum pernix K1
-
2-oxoglutarate + NADPH + NH3
-
r

Organism

Organism UniProt Comment Textmining
Aeropyrum pernix K1
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH3 + NADH + H+ NH4Cl used in enzyme assay Aeropyrum pernix K1 L-glutamate + H2O + NAD+
-
r
L-glutamate + NADP+ + H2O
-
Aeropyrum pernix K1 2-oxoglutarate + NADPH + NH3
-
r

Subunits

Subunits Comment Organism
homohexamer alpha6, 6 * 47000, SDS-PAGE Aeropyrum pernix K1

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
reductive amination Aeropyrum pernix K1
95
-
oxidative deamination Aeropyrum pernix K1

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
100
-
74% activity after 5 h Aeropyrum pernix K1

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
reductive amination and oxidative deamination Aeropyrum pernix K1

pH Range

pH Minimum pH Maximum Comment Organism
4 9 above pH 9 or below 4.0 no activity Aeropyrum pernix K1

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Aeropyrum pernix K1
NADPH
-
Aeropyrum pernix K1