Literature summary for 1.4.1.4 extracted from

Lebbink, J.H.; Knapp, S.; van der Oost, J.; Rice, D.; Ladenstein, R.; de Vos, W.M.
Engineering activity and stability of Thermotoga maritima glutamate dehydrogenase. II: construction of a 16-residue ion-pair network at the subunit interface (1999), J. Mol. Biol., 289, 357-369.

Search for more literature for 1.4.1.4

Crystallization (Commentary)

Crystallization (Comment)	Organism
quadruple mutant S128R/T158E/N117R/S160E, homohexamer, 2.9 A resolution	Thermotoga maritima

Protein Variants

Protein Variants	Comment	Organism
N117R	80% activity of wild-type at optimum temperature for catalysis	Thermotoga maritima
S128R	same activity as wild-type at optimum temperature for catalysis	Thermotoga maritima
S128R/T158E	120% activity of wild-type at optimum temperature for catalysis	Thermotoga maritima
S128R/T158E/N117R	same activity as wild-type at optimum temperature for catalysis	Thermotoga maritima
S128R/T158E/N117R/S160E	same activity as wild-type at optimum temperature for catalysis	Thermotoga maritima
S128R/T158E/S160E	same activity as wild-type at optimum temperature for catalysis	Thermotoga maritima
T158E	60% activity of wild-type at optimum temperature for catalysis	Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-glutamate + NADP+ + H2O	Thermotoga maritima	-	2-oxoglutarate + NADPH + NH3	-	r

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.5	-	-	Thermotoga maritima

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-glutamate + NADP+ + H2O	-	Thermotoga maritima	2-oxoglutarate + NADPH + NH3	-	r

Subunits

Subunits	Comment	Organism
homohexamer	alpha6, 6 * ?, crystallization	Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
55	-	T158E-mutant	Thermotoga maritima
58	-	S128R-mutant	Thermotoga maritima
60	-	S128R/T158E/S160E-mutant	Thermotoga maritima
63	-	N117R-mutant and S128R/T158E/N117R/S160E-mutant	Thermotoga maritima
65	-	wild-type and S128R/T158E-mutant	Thermotoga maritima
68	-	S128R/T158E/N117R-mutant	Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
85	-	wild-type 50% activity after 209 min	Thermotoga maritima
85	-	quadruple mutant 50% activity after 5 min	Thermotoga maritima

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Thermotoga maritima
NADPH	-	Thermotoga maritima

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Release 2023.1 (January 2023)