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Literature summary for 1.4.1.4 extracted from

  • Hemmilä, I.A.; Mäntsälä, P.I.
    Purification and properties of glutamate synthase and glutamate dehydrogenase from Bacillus megaterium (1978), Biochem. J., 173, 45-52.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.4.1.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0087
-
 NADPH reductive amination Priestia megaterium
0.05
-
 NADP+ oxidative deamination Priestia megaterium
0.36
-
 2-oxoglutarate reductive amination Priestia megaterium
22
-
 NH4+ reductive amination Priestia megaterium
29
-
 L-glutamate oxidative deamination Priestia megaterium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 alpha6, 6 * 47000, SDS-PAGE Priestia megaterium
270000
-
 sucrose-density-gradient centrifugation Priestia megaterium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + NADP+ + H2O Priestia megaterium
-
 2-oxoglutarate + NADPH + NH3
-
 r

Organism

Organism UniProt Comment Textmining
Priestia megaterium
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NH4+ + NADPH
-
 Priestia megaterium L-glutamate + NADP+
-
 ?
L-glutamate + NADP+ + H2O
-
 Priestia megaterium 2-oxoglutarate + NADPH + NH3
-
 r

Subunits

Subunits Comment Organism
homohexamer alpha6, 6 * 47000, SDS-PAGE Priestia megaterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
 reductive amination Priestia megaterium
9
-
 oxidative deamination Priestia megaterium

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Priestia megaterium
NADPH
-
 Priestia megaterium