Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | Arabidopsis thaliana | - |
2-oxoglutarate + NH3 + NADH + H+ | - |
r | |
L-glutamate + H2O + NADP+ | Arabidopsis thaliana | - |
2-oxoglutarate + NH3 + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q43314 AND Q38946 | alpha and beta subunits, encoded by GDH1 and GDH2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
mesophyll | - |
Arabidopsis thaliana | - |
vascular bundle | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | - |
Arabidopsis thaliana | 2-oxoglutarate + NH3 + NADH + H+ | - |
r | |
L-glutamate + H2O + NADP+ | - |
Arabidopsis thaliana | 2-oxoglutarate + NH3 + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
At5g07440 | gene name, beta-subunit | Arabidopsis thaliana |
At5g18170 | gene name, alpha-subunit | Arabidopsis thaliana |
GDH1 | gene name, alpha-subunit | Arabidopsis thaliana |
GDH2 | gene name, beta-subunit | Arabidopsis thaliana |
NADH-GDH | - |
Arabidopsis thaliana |
NADH-glutamate dehydrogenase | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Arabidopsis thaliana | |
NADH | - |
Arabidopsis thaliana | |
NADP+ | - |
Arabidopsis thaliana | |
NADPH | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in the nitrogen distribution during primary assimilation, photorespiratory re-assimilation and translocation in Arabidopsis thaliana, overview. Traditional route of glutamate formation via the 2-oxoglutarate amination with NH4+ is persued by mitochondrial NADH-glutamate dehydrogenase | Arabidopsis thaliana |
physiological function | in vitro, GDH catalyzes the reversible deamination of Glu to NH4 +, 2-oxoglutarate forming NADH. The reversible lethal phenotype of gltS mutants reveals that mitochondrial NADH-GDH is unable to re-assimilate photorespiratory NH3 produced within the same organelle although its amination activity. In vitro is several fold higher than the glutamate synthase isozyme Fd-GOGAT (EC 1.4.7.1) activity. The in vivo direction of reversible NADH-GDH reaction is controversial. NADH-GDH can exist as two homohexamers of either alpha subunit encoded by GDH1 or beta subunit encoded by GDH2 and five heterohexamers containing both alpha and beta subunits in Arabidopsis thaliana as observed in other plants | Arabidopsis thaliana |