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Literature summary for 1.4.1.3 extracted from

  • Zaganas, I.; Pajecka, K.; Wendel Nielsen, C.; Schousboe, A.; Waagepetersen, H.S.; Plaitakis, A.
    The effect of pH and ADP on ammonia affinity for human glutamate dehydrogenases (2013), Metab. Brain Dis., 28, 127-131.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of wild-type GLUD1 and GLUD2 in Spodoptera frugiperda Sf21 cells using the baculovirus expression system Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information lowering the pH of the buffer from pH 8.0 to pH 7.0 increases the Km for ammonia substantially, i.e. for hGDH1 from 12.8 mM to 57.5 mM, and for hGDH2: from 14.7 mM to 62.2 mM, thus essentially precluding reductive amination Homo sapiens
12.8
-
NH3 hGDH1, pH 8.0, temperature not specified in the publication Homo sapiens
14.7
-
NH3 hGDH2, pH 8.0, temperature not specified in the publication Homo sapiens
33
-
NH3 hGDH2, pH 7.5, temperature not specified in the publication Homo sapiens
35
-
NH3 hGDH1, pH 7.5, temperature not specified in the publication Homo sapiens
57.5
-
NH3 hGDH1, pH 7.0, temperature not specified in the publication Homo sapiens
62.2
-
NH3 hGDH2, pH 7.0, temperature not specified in the publication Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + H2O + NAD+ Homo sapiens
-
2-oxoglutarate + NH3 + NADH + H+
-
r
L-glutamate + H2O + NADP+ Homo sapiens
-
2-oxoglutarate + NH3 + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
two human isozymes hGDH1 and hGDH2, genes GLUD1 and GLUD2
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type GLUD1 and GLUD2 from Spodoptera frugiperda Sf21 cells by ammonim sulfate fractionation, and hydrophobic interaction and hydroxyapatite chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD+
-
Homo sapiens 2-oxoglutarate + NH3 + NADH + H+
-
r
L-glutamate + H2O + NADP+
-
Homo sapiens 2-oxoglutarate + NH3 + NADPH + H+
-
r

Synonyms

Synonyms Comment Organism
GDH
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
higher activity than at pH 7.0 Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7 8 lowering the pH of the buffer from pH 8.0 to pH 7.0 increases the Km for ammonia substantially, i.e. for hGDH1 from 12.8 mM to 57.5 mM, and for hGDH2: from 14.7 mM to 62.2 mM, thus essentially precluding reductive amination Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Homo sapiens
NADH
-
Homo sapiens
NADP+
-
Homo sapiens
NADPH
-
Homo sapiens

General Information

General Information Comment Organism
evolution while GDH in most mammals is encoded by a single GLUD1 gene, humans and other primates have acquired a GLUD2 gene with distinct tissue expression profile Homo sapiens