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Literature summary for 1.4.1.3 extracted from

  • Klump, H.; Di Ruggiero, J.; Kessel, M.; Park, J.B.; Adams, M.W.; Robb, F.T.
    Glutamate dehydrogenase from the hyperthermophile Pyrococcus furiosus. Thermal denaturation and activation (1992), J. Biol. Chem., 267, 22681-22685.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
270000
-
-
Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + H2O + NAD(P)+ Pyrococcus furiosus
-
2-oxoglutarate + NH3 + NAD(P)H + H+
-
?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD(P)+
-
Pyrococcus furiosus 2-oxoglutarate + NH3 + NAD(P)H + H+
-
?
L-glutamate + H2O + NAD+
-
Pyrococcus furiosus 2-oxoglutarate + NH3 + NADH + H+
-
?
L-glutamate + H2O + NADP+
-
Pyrococcus furiosus 2-oxoglutarate + NH3 + NADPH + H+
-
?

Subunits

Subunits Comment Organism
hexamer
-
Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85
-
assay at Pyrococcus furiosus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40
-
inactive at temperatures below 40°C, undergoes heat activation above 40°C Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
110
-
thermal denaturation starts at 110°C and is completed at 118°C. The process of heat activation from 40 to 80°C is accompanied by a much smaller increase in absorbance at 280 nm and a reversible increase in heat capacity with DELTAcal = 187 Kcal/mol GDH and Tm = 57°C. This absorbance change as well as the moderate increase in heat capacity suggest that thermal activation leads to some exposure of hydrophobic groups to solvent water as the GDH structure is opened slightly. The increase in absorbance at 280 nm during activation is only 12% of that for denaturation Pyrococcus furiosus
113
-
Tm-value = 113°C Pyrococcus furiosus
118
-
thermal denaturation starts at 110°C and is comp1eted at 118°C Pyrococcus furiosus

Cofactor

Cofactor Comment Organism Structure
NAD+ both NAD+ and NADP+ are utilized as cofactors. The inactive enzyme also binds cofactors Pyrococcus furiosus
NADP+ both NAD+ and NADP+ are utilized as cofactors. The inactive enzyme also binds cofactors Pyrococcus furiosus