Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | positive allosteric regulator | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
gene GLUD2, expression of 12-amino acid GDH2-specific peptide PTAEFQDSISGA, corresponding to residues 436-447 of the mature human protein, in Spodoptere frugiperda Sf21 cells using the baculovirus transfection method | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
Q441R | site-directed mutagenesis, the specific antibody, generated from 12-amino acid hGDH2-specific peptide PTAEFQDSISGA, corresponding to residues 436-447 of the mature human protein, shows reduced reactivity with the enzyme mutant | Homo sapiens |
S445A | site-directed mutagenesis, the specific antibody, generated from 12-amino acid hGDH2-specific peptide PTAEFQDSISGA, corresponding to residues 436-447 of the mature human protein, shows reduced reactivity with the enzyme mutant | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
GTP | negative allosteric regulator | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | GDH2 | Homo sapiens | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | Homo sapiens | - |
2-oxoglutarate + NH3 + NADH + H+ | - |
r | |
L-glutamate + H2O + NADP+ | Homo sapiens | - |
2-oxoglutarate + NH3 + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P49448 | gene GLUD2 encoding isozyme GDH2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
astrocyte | high GDH2 expression level | Homo sapiens | - |
brain | - |
Homo sapiens | - |
cerebral cortex | high expression in astrocytes, low expression in neurons | Homo sapiens | - |
additional information | GDH2 tissue distribution, immunohistochemic analysis using a specific antibody generated with the 12-amino acid hGDH2-specific peptide PTAEFQDSISGA, corresponding to residues 436-447 of the mature human protein, overview | Homo sapiens | - |
retina | - |
Homo sapiens | - |
Sertoli cell | high GDH2 expression level | Homo sapiens | - |
testis | mainly in Sertoli cells | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | - |
Homo sapiens | 2-oxoglutarate + NH3 + NADH + H+ | - |
r | |
L-glutamate + H2O + NADP+ | - |
Homo sapiens | 2-oxoglutarate + NH3 + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
GDH2 | - |
Homo sapiens |
glutamate dehydrogenase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens | |
NADH | - |
Homo sapiens | |
NADP+ | - |
Homo sapiens | |
NADPH | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | the glutamate dehydrogenase catalyzes the reversible interconversion of glutamate to 2-oxoglutarate and ammonia using NADP(H) and NAD(H) as cofactors, thus interconnecting amino acid and carbohydrate metabolism. Mammalian GDH is allosterically regulated, with GTP and ADP being the main negative and positive modulators, respectively | Homo sapiens |