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Literature summary for 1.4.1.3 extracted from

  • Spanaki, C.; Zaganas, I.; Kleopa, K.A.; Plaitakis, A.
    Human GLUD2 glutamate dehydrogenase is expressed in neural and testicular supporting cells (2010), J. Biol. Chem., 285, 16748-16756.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
ADP positive allosteric regulator Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
gene GLUD2, expression of 12-amino acid GDH2-specific peptide PTAEFQDSISGA, corresponding to residues 436-447 of the mature human protein, in Spodoptere frugiperda Sf21 cells using the baculovirus transfection method Homo sapiens

Protein Variants

Protein Variants Comment Organism
Q441R site-directed mutagenesis, the specific antibody, generated from 12-amino acid hGDH2-specific peptide PTAEFQDSISGA, corresponding to residues 436-447 of the mature human protein, shows reduced reactivity with the enzyme mutant Homo sapiens
S445A site-directed mutagenesis, the specific antibody, generated from 12-amino acid hGDH2-specific peptide PTAEFQDSISGA, corresponding to residues 436-447 of the mature human protein, shows reduced reactivity with the enzyme mutant Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
GTP negative allosteric regulator Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion GDH2 Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + H2O + NAD+ Homo sapiens
-
2-oxoglutarate + NH3 + NADH + H+
-
r
L-glutamate + H2O + NADP+ Homo sapiens
-
2-oxoglutarate + NH3 + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Homo sapiens P49448 gene GLUD2 encoding isozyme GDH2
-

Source Tissue

Source Tissue Comment Organism Textmining
astrocyte high GDH2 expression level Homo sapiens
-
brain
-
Homo sapiens
-
cerebral cortex high expression in astrocytes, low expression in neurons Homo sapiens
-
additional information GDH2 tissue distribution, immunohistochemic analysis using a specific antibody generated with the 12-amino acid hGDH2-specific peptide PTAEFQDSISGA, corresponding to residues 436-447 of the mature human protein, overview Homo sapiens
-
retina
-
Homo sapiens
-
Sertoli cell high GDH2 expression level Homo sapiens
-
testis mainly in Sertoli cells Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD+
-
Homo sapiens 2-oxoglutarate + NH3 + NADH + H+
-
r
L-glutamate + H2O + NADP+
-
Homo sapiens 2-oxoglutarate + NH3 + NADPH + H+
-
r

Synonyms

Synonyms Comment Organism
GDH2
-
Homo sapiens
glutamate dehydrogenase
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Homo sapiens
NADH
-
Homo sapiens
NADP+
-
Homo sapiens
NADPH
-
Homo sapiens

General Information

General Information Comment Organism
physiological function the glutamate dehydrogenase catalyzes the reversible interconversion of glutamate to 2-oxoglutarate and ammonia using NADP(H) and NAD(H) as cofactors, thus interconnecting amino acid and carbohydrate metabolism. Mammalian GDH is allosterically regulated, with GTP and ADP being the main negative and positive modulators, respectively Homo sapiens