Cloned (Comment) | Organism |
---|---|
wild-type enzyme hGDH2 and mutant enzymes | Homo sapiens |
wild-type hGDH1 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A456G | mutant of isoenzyme hGDH2 shows no change in heat inactivation process compared to wild-type enzyme | Homo sapiens |
H470R | mutant of isoenzyme hGDH2 shows no change in heat inactivation process compared to wild-type enzyme | Homo sapiens |
L415M | mutant of isoenzyme hGDH2 shows no change in heat inactivation process compared to wild-type enzyme | Homo sapiens |
S443R | mutant of isoenzyme hGDH2 shows a dramatic increase in thermal stability from 45 min at 45°C for the wild-type enzyme to 300 min for the mutant enzyme. KM-values and turnover-numbers are nearly identical to wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.081 | - |
NADH | pH 8.0, 25°C, wild-type enzyme hGDH1 | Homo sapiens | |
0.086 | - |
NADH | pH 8.0, 25°C, wild-type enzyme hGDH2 | Homo sapiens | |
0.088 | - |
NADH | pH 8.0, 25°C, mutant enzyme S443R hGDH2 | Homo sapiens | |
1.25 | - |
2-oxoglutarate | pH 8.0, 25°C, wild-type enzyme hGDH1 | Homo sapiens | |
1.39 | - |
2-oxoglutarate | pH 8.0, 25°C, wild-type enzyme hGDH2 | Homo sapiens | |
1.41 | - |
2-oxoglutarate | pH 8.0, 25°C, mutant enzyme S443R hGDH2 | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56500 | - |
6 * 56500, hGDH1, SDS-PAGE | Homo sapiens |
56500 | - |
6 * 56500, hGDH2, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxoglutarate + NADH + NH3 | - |
Homo sapiens | L-glutamate + NAD+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 56500, hGDH1, SDS-PAGE | Homo sapiens |
hexamer | 6 * 56500, hGDH2, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
hGDH1 | - |
Homo sapiens |
hGDH2 | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
hGDH1: much slower heat inactivation processes in presence of 1 mM ADP or 3 mM L-Leu | Homo sapiens |
additional information | - |
much slower heat inactivation processes in presence of 1 mM ADP or 3 mM L-Leu | Homo sapiens |
45 | - |
half-life of hGDH1 is 310 min in absence of allosteric regulators | Homo sapiens |
45 | - |
half-life of hGDH2 is 45 min in absence of allosteric regulators, hGDH2 is stable for 100 min in presence of 3 mM L-Leu and 1 mM ADP, mutant enzyme hGDH2 S443R has a half life of 300 min | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
104 | - |
NADH | pH 8.0, 25°C, wild-type enzyme hGDH1 | Homo sapiens | |
104 | - |
2-oxoglutarate | pH 8.0, 25°C, wild-type enzyme hGDH1 | Homo sapiens | |
130 | - |
NADH | pH 8.0, 25°C, wild-type enzyme hGDH2 | Homo sapiens | |
130 | - |
2-oxoglutarate | pH 8.0, 25°C, wild-type enzyme hGDH2 | Homo sapiens | |
134 | - |
NADH | pH 8.0, 25°C, mutant enzyme S443R hGDH2 | Homo sapiens | |
134 | - |
2-oxoglutarate | pH 8.0, 25°C, mutant enzyme S443R hGDH2 | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Homo sapiens |