Cloned (Comment) | Organism |
---|---|
expression by in vitro transcription/translation in rabbit reticulocytes | Pyrococcus sp. |
General Stability | Organism |
---|---|
absolutely stable at 4°C or at room temperature for at least 6 months | Pyrococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
46000 | - |
6 * 46000, SDS-PAGE | Pyrococcus sp. |
270000 | - |
gel filtration | Pyrococcus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus sp. | - |
ES4, hyperthermophilic archeon | - |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD(P)+ | - |
Pyrococcus sp. | 2-oxoglutarate + NH3 + NAD(P)H | - |
r |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 46000, SDS-PAGE | Pyrococcus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | 105 | 1 mg/ml enzyme, half-life of 10.5 h at 100°C, 3.5 h at 105°C, and 20 h at 90°C, thermal denaturation at 113°C | Pyrococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | specific for | Pyrococcus sp. |