Application | Comment | Organism |
---|---|---|
synthesis | in the asymmetric amination of a series of structurally different hydroxy ketones, high stereoselectivity (above >99%) is achieved the reduction of aliphatic and aromatic compounds. The aliphatic substrates 1-hydroxy-4-methylpentan-2-one and 1-hydroxyhexan-2-one are aminated with 99% conversions | Geobacillus stearothermophilus |
Crystallization (Comment) | Organism |
---|---|
homology modeling of structure in complex with NADH. Residue S68 of AmDH forms a hydrogen bond with the substrate hydroxyl group, and there are also hydrophobic interactions between the substrate and the side chains of V116 and L261 | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.48 | - |
1-hydroxybutan-2-one | pH 8.5, 50°C | Geobacillus stearothermophilus | |
2.32 | - |
1-hydroxybutan-2-one | pH 9.0, 55°C | Geobacillus stearothermophilus | |
2.42 | - |
1-hydroxybutan-2-one | pH 8.5, 50°C | Sporosarcina psychrophila | |
3.44 | - |
1-hydroxybutan-2-one | pH 8.5, 55°C | Lysinibacillus sphaericus | |
3.64 | - |
1-hydroxybutan-2-one | pH 8.5, 45°C | Thermoactinomyces intermedius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
- |
- |
Geobacillus stearothermophilus | P13154 | cf. EC 1.4.1.9 | - |
Lysinibacillus sphaericus | W7S058 | cf. EC 1.4.1.9 | - |
Lysinibacillus sphaericus CBAM5 | W7S058 | cf. EC 1.4.1.9 | - |
Sporosarcina psychrophila | I0IJU1 | cf. EC 1.4.1.9 | - |
Thermoactinomyces intermedius | Q60030 | cf. EC 1.4.1.9 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-hydroxy-4-methylpentan-2-one + NH3 + NADH + H+ | - |
Geobacillus stearothermophilus | 2-amino-4-methylpentan-1-ol + H2O + NAD+ | - |
? | |
1-hydroxybutan-2-one + NH3 + NAD(P)H + H+ | - |
Geobacillus stearothermophilus | (S)-2-aminobutan-1-ol + H2O + NAD(P)+ | - |
? | |
1-hydroxybutan-2-one + NH3 + NAD(P)H + H+ | - |
Lysinibacillus sphaericus | (S)-2-aminobutan-1-ol + H2O + NAD(P)+ | - |
? | |
1-hydroxybutan-2-one + NH3 + NAD(P)H + H+ | - |
Sporosarcina psychrophila | (S)-2-aminobutan-1-ol + H2O + NAD(P)+ | - |
? | |
1-hydroxybutan-2-one + NH3 + NAD(P)H + H+ | - |
Thermoactinomyces intermedius | (S)-2-aminobutan-1-ol + H2O + NAD(P)+ | - |
? | |
1-hydroxybutan-2-one + NH3 + NAD(P)H + H+ | - |
Lysinibacillus sphaericus CBAM5 | (S)-2-aminobutan-1-ol + H2O + NAD(P)+ | - |
? | |
1-hydroxyhexan-2-one + NH3 + NADH + H+ | - |
Geobacillus stearothermophilus | (S)-2-aminohexan-1-ol + H2O + NAD+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Thermoactinomyces intermedius |
50 | - |
- |
Geobacillus stearothermophilus |
55 | - |
- |
Geobacillus stearothermophilus |
55 | - |
- |
Lysinibacillus sphaericus |
55 | - |
- |
Sporosarcina psychrophila |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
1-hydroxybutan-2-one | pH 8.5, 45°C | Thermoactinomyces intermedius | |
0.74 | - |
1-hydroxybutan-2-one | pH 8.5, 50°C | Sporosarcina psychrophila | |
0.77 | - |
1-hydroxybutan-2-one | pH 8.5, 50°C | Geobacillus stearothermophilus | |
1.52 | - |
1-hydroxybutan-2-one | pH 8.5, 55°C | Lysinibacillus sphaericus | |
1.79 | - |
1-hydroxybutan-2-one | pH 9.0, 55°C | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Geobacillus stearothermophilus |
8.5 | - |
- |
Lysinibacillus sphaericus |
8.5 | - |
- |
Sporosarcina psychrophila |
8.5 | - |
- |
Thermoactinomyces intermedius |
9 | - |
- |
Geobacillus stearothermophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
1-hydroxybutan-2-one | pH 8.5, 45°C | Thermoactinomyces intermedius | |
0.31 | - |
1-hydroxybutan-2-one | pH 8.5, 50°C | Sporosarcina psychrophila | |
0.44 | - |
1-hydroxybutan-2-one | pH 8.5, 55°C | Lysinibacillus sphaericus | |
0.52 | - |
1-hydroxybutan-2-one | pH 8.5, 50°C | Geobacillus stearothermophilus | |
0.71 | - |
1-hydroxybutan-2-one | pH 9.0, 55°C | Geobacillus stearothermophilus |