Crystallization (Comment) | Organism |
---|---|
structure without substrate but bound to the cofactor NAD+, and a model of the ternary complex with product (2R,4S)-2,4-diaminopentanoate in the closed conformation. The active site features residues Glu102, Val134, Asn135, Arg161, Asn163, Phe168, Val172, Gln176, His197, Ile198, His264, Gln266, Gly299 and Thr303 | Petrotoga mobilis |
Protein Variants | Comment | Organism |
---|---|---|
E102A | residue Glu102 is involved in the activation of ammonia, mutation significantly decreases activity | Petrotoga mobilis |
R161M | mutant gains activity with substrate pentan-2-one | Petrotoga mobilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-methylcyclohexanone | - |
Microbacterium sp. MA1 | |
2-methylcyclohexanone | - |
Mycolicibacterium smegmatis | |
2-methylcyclohexanone | - |
Petrotoga mobilis | |
cyclohexanone | substrate inhibition | Cystobacter fuscus | |
cyclohexanone | - |
Microbacterium sp. MA1 | |
cyclohexanone | - |
Mycolicibacterium smegmatis | |
cyclohexanone | - |
Petrotoga mobilis | |
Isobutanal | - |
Microbacterium sp. MA1 | |
Isobutanal | - |
Mycolicibacterium smegmatis | |
Isobutanal | - |
Petrotoga mobilis | |
NH3 | substrate inhibition | Cystobacter fuscus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NADPH | pH 9.5, 23°C | Cystobacter fuscus | |
0.025 | - |
NADH | wild-type, pH 9.5, 23°C | Petrotoga mobilis | |
0.06 | - |
NADPH | pH 9.5, 23°C | Mycolicibacterium smegmatis | |
0.06 | - |
NADH | pH 9.5, 23°C | Cystobacter fuscus | |
0.29 | - |
NADH | pH 9.5, 23°C | Mycolicibacterium smegmatis | |
0.326 | - |
(2R)-2-amino-4-oxopentanoate | wild-type, pH 9.5, 23°C | Petrotoga mobilis | |
0.54 | - |
Isobutanal | pH 9.5, 23°C | Cystobacter fuscus | |
0.55 | - |
cyclohexanone | pH 9.5, 23°C | Mycolicibacterium smegmatis | |
0.86 | - |
Isobutanal | pH 9.5, 23°C | Mycolicibacterium smegmatis | |
0.894 | - |
(2R)-2-amino-4-oxopentanoate | mutant E102A, pH 9.5, 23°C | Petrotoga mobilis | |
2.32 | - |
2-methylcyclohexanone | pH 9.5, 23°C | Mycolicibacterium smegmatis | |
2.47 | - |
cyclohexanone | pH 9.5, 23°C | Cystobacter fuscus | |
98.94 | - |
NH3 | pH 9.5, 23°C | Cystobacter fuscus | |
319.7 | - |
NH3 | wild-type, pH 9.5, 23°C | Petrotoga mobilis | |
392.2 | - |
NH3 | pH 9.5, 23°C | Mycolicibacterium smegmatis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R)-2-amino-4-oxopentanoate + NH3 + NADH + H+ | Petrotoga mobilis | - |
(2R,4S)-2,4-diaminopentanoate + NAD+ + H2O | - |
? | |
(2R)-2-amino-4-oxopentanoate + NH3 + NADH + H+ | Petrotoga mobilis DSM 10674 | - |
(2R,4S)-2,4-diaminopentanoate + NAD+ + H2O | - |
? | |
pentan-2-one + NH3 + NADH + H+ | Petrotoga mobilis | no substrate of wild-type, but substrate of mutants carrying substitution R161M | pentan-2-amine + NAD+ + H2O | - |
? | |
pentan-2-one + NH3 + NADH + H+ | Petrotoga mobilis DSM 10674 | no substrate of wild-type, but substrate of mutants carrying substitution R161M | pentan-2-amine + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cystobacter fuscus | S9Q235 | - |
- |
Cystobacter fuscus DSM 2262 | S9Q235 | - |
- |
Microbacterium sp. MA1 | C3UMY1 | - |
- |
Mycolicibacterium smegmatis | A0A8B4R7U8 | - |
- |
Petrotoga mobilis | A9BHL2 | - |
- |
Petrotoga mobilis DSM 10674 | A9BHL2 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10.1 | - |
substrate cyclohexanone, cosubstrate methylamine, pH 9.5, 23°C | Mycolicibacterium smegmatis |
16.4 | - |
substrate 2-methylbutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C | Mycolicibacterium smegmatis |
18.6 | - |
substrate 3-methylbutan-2-one, cosubstrates NH3 and NADPH, pH 9.5, 23°C | Mycolicibacterium smegmatis |
19.6 | - |
substrate 2-methylcyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C | Cystobacter fuscus |
25.1 | - |
substrate cyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C | Mycolicibacterium smegmatis |
25.5 | - |
substrate 2-methylcyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C | Cystobacter fuscus |
37.3 | - |
substrate 2-methylcyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C | Mycolicibacterium smegmatis |
54.4 | - |
substrate 3-methylbutan-2-one, cosubstrates NH3 and NADPH, pH 9.5, 23°C | Microbacterium sp. MA1 |
60.2 | - |
substrate cyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C | Cystobacter fuscus |
73.8 | - |
substrate isobutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C | Cystobacter fuscus |
75.2 | - |
substrate 2-methylbutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C | Mycolicibacterium smegmatis |
75.2 | - |
substrate 2-methylbutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C | Microbacterium sp. MA1 |
88.1 | - |
substrate isobutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C | Mycolicibacterium smegmatis |
88.1 | - |
substrate isobutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C | Microbacterium sp. MA1 |
90.4 | - |
substrate cyclohexanone, cosubstrate methylamine, pH 9.5, 23°C | Microbacterium sp. MA1 |
97.6 | - |
substrate 2-methylbutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C | Microbacterium sp. MA1 |
104.8 | - |
mutant N135V/N163V/R161M/H264L, substrate pentan-2-one, 23°C, pH 9.5 | Petrotoga mobilis |
109.6 | - |
substrate 3-methylbutan-2-one, cosubstrates NH3 and NADPH, pH 9.5, 23°C | Cystobacter fuscus |
119.2 | - |
substrate isobutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C | Mycolicibacterium smegmatis |
120.6 | - |
substrate 3-methylbutan-2-one, cosubstrates NH3 and NADPH, pH 9.5, 23°C | Cystobacter fuscus |
129 | - |
substrate 3-methylbutan-2-one, cosubstrates NH3 and NADPH, pH 9.5, 23°C | Mycolicibacterium smegmatis |
131.6 | - |
substrate 2-methylbutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C | Cystobacter fuscus |
144.5 | - |
substrate isobutanal, cosubstrate methylamine, pH 9.5, 23°C | Mycolicibacterium smegmatis |
159.4 | - |
substrate isobutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C | Microbacterium sp. MA1 |
175.2 | - |
substrate 2-methylcyclohexanone, cosubstrates NH3 andNADPH, pH 9.5, 23°C | Mycolicibacterium smegmatis |
196.2 | - |
substrate cyclohexanone, cosubstrates NH3 and NADH, pH 9.5, 23°C | Mycolicibacterium smegmatis |
233.2 | - |
substrate cyclohexanone, cosubstrates NH3 and NADH, pH 9.5, 23°C | Cystobacter fuscus |
245.6 | - |
substrate 3-methylbutan-2-one, cosubstrates NH3 and NADPH, pH 9.5, 23°C | Microbacterium sp. MA1 |
252.6 | - |
substrate 2-methylcyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C | Microbacterium sp. MA1 |
257.7 | - |
substrate isobutanal, cosubstrate methylamine, pH 9.5, 23°C | Cystobacter fuscus |
337 | - |
substrate 2-methylcyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C | Microbacterium sp. MA1 |
388.6 | - |
substrate cyclohexanone, cosubstrates NH3 and NADPH, pH 9.5, 23°C | Microbacterium sp. MA1 |
397.9 | - |
substrate 2-methylbutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C | Cystobacter fuscus |
614.5 | - |
substrate cyclohexanone, cosubstrates NH3 and NADH, pH 9.5, 23°C | Microbacterium sp. MA1 |
851.3 | - |
substrate isobutanal, cosubstrate methylamine, pH 9.5, 23°C | Microbacterium sp. MA1 |
955 | - |
substrate isobutanal, cosubstrates NH3 and NADPH, pH 9.5, 23°C | Cystobacter fuscus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R)-2-amino-4-oxopentanoate + NH3 + NADH + H+ | - |
Petrotoga mobilis | (2R,4S)-2,4-diaminopentanoate + NAD+ + H2O | - |
? | |
(2R)-2-amino-4-oxopentanoate + NH3 + NADH + H+ | - |
Petrotoga mobilis DSM 10674 | (2R,4S)-2,4-diaminopentanoate + NAD+ + H2O | - |
? | |
1-cyclohexylethan-1-one + NH3 + NADH + H+ | - |
Mycolicibacterium smegmatis | 1-cyclohexylethyl-1-amine + NAD+ + H2O | - |
? | |
1-cyclohexylethan-1-one + NH3 + NADH + H+ | - |
Cystobacter fuscus | 1-cyclohexylethyl-1-amine + NAD+ + H2O | - |
? | |
1-cyclohexylethan-1-one + NH3 + NADH + H+ | - |
Microbacterium sp. MA1 | 1-cyclohexylethyl-1-amine + NAD+ + H2O | - |
? | |
1-cyclohexylethan-1-one + NH3 + NADH + H+ | - |
Cystobacter fuscus DSM 2262 | 1-cyclohexylethyl-1-amine + NAD+ + H2O | - |
? | |
1-indanone + NH3 + NADH + H+ | - |
Mycolicibacterium smegmatis | 1-indanamine + NAD+ + H2O | - |
? | |
1-indanone + NH3 + NADH + H+ | - |
Cystobacter fuscus | 1-indanamine + NAD+ + H2O | - |
? | |
1-indanone + NH3 + NADH + H+ | - |
Microbacterium sp. MA1 | 1-indanamine + NAD+ + H2O | - |
? | |
1-indanone + NH3 + NADH + H+ | - |
Cystobacter fuscus DSM 2262 | 1-indanamine + NAD+ + H2O | - |
? | |
2-cyclohexen-1-one + NH3 + NADH + H+ | - |
Mycolicibacterium smegmatis | 2-cyclohexen-1-amine + NAD+ + H2O | - |
? | |
2-cyclohexen-1-one + NH3 + NADH + H+ | - |
Cystobacter fuscus | 2-cyclohexen-1-amine + NAD+ + H2O | - |
? | |
2-cyclohexen-1-one + NH3 + NADH + H+ | - |
Microbacterium sp. MA1 | 2-cyclohexen-1-amine + NAD+ + H2O | - |
? | |
2-cyclohexen-1-one + NH3 + NADH + H+ | - |
Cystobacter fuscus DSM 2262 | 2-cyclohexen-1-amine + NAD+ + H2O | - |
? | |
2-methylbutanal + NH3 + NADH + H+ | - |
Mycolicibacterium smegmatis | 2-methylbutan-1-amine + NAD+ + H2O | - |
? | |
2-methylbutanal + NH3 + NADH + H+ | - |
Cystobacter fuscus | 2-methylbutan-1-amine + NAD+ + H2O | - |
? | |
2-methylbutanal + NH3 + NADH + H+ | - |
Microbacterium sp. MA1 | 2-methylbutan-1-amine + NAD+ + H2O | - |
? | |
2-methylbutanal + NH3 + NADH + H+ | - |
Cystobacter fuscus DSM 2262 | 2-methylbutan-1-amine + NAD+ + H2O | - |
? | |
2-methylcyclohexanone + NH3 + NADH + H+ | - |
Mycolicibacterium smegmatis | 2-methylcyclohexylamine + NAD+ + H2O | - |
? | |
2-methylcyclohexanone + NH3 + NADH + H+ | - |
Cystobacter fuscus | 2-methylcyclohexylamine + NAD+ + H2O | - |
? | |
2-methylcyclohexanone + NH3 + NADH + H+ | - |
Microbacterium sp. MA1 | 2-methylcyclohexylamine + NAD+ + H2O | - |
? | |
2-methylcyclopentanone + NH3 + NADH + H+ | - |
Mycolicibacterium smegmatis | 2-methylcyclopentylamine + NAD+ + H2O | - |
? | |
2-methylcyclopentanone + NH3 + NADH + H+ | - |
Cystobacter fuscus | 2-methylcyclopentylamine + NAD+ + H2O | - |
? | |
2-methylcyclopentanone + NH3 + NADH + H+ | - |
Microbacterium sp. MA1 | 2-methylcyclopentylamine + NAD+ + H2O | - |
? | |
3,5-dimethylcyclohexanone + NH3 + NADH + H+ | - |
Mycolicibacterium smegmatis | 3,5-dimethylcyclohexylamine + NAD+ + H2O | - |
? | |
3,5-dimethylcyclohexanone + NH3 + NADH + H+ | - |
Cystobacter fuscus | 3,5-dimethylcyclohexylamine + NAD+ + H2O | - |
? | |
3,5-dimethylcyclohexanone + NH3 + NADH + H+ | - |
Microbacterium sp. MA1 | 3,5-dimethylcyclohexylamine + NAD+ + H2O | - |
? | |
3-hydroxybutan-2-one + NH3 + NADH + H+ | - |
Mycolicibacterium smegmatis | 3-aminobutan-2-ol + NAD+ + H2O | - |
? | |
3-hydroxybutan-2-one + NH3 + NADH + H+ | - |
Cystobacter fuscus | 3-aminobutan-2-ol + NAD+ + H2O | - |
? | |
3-hydroxybutan-2-one + NH3 + NADH + H+ | - |
Microbacterium sp. MA1 | 3-aminobutan-2-ol + NAD+ + H2O | - |
? | |
3-methylbutan-2-one + NH3 + NADH + H+ | - |
Mycolicibacterium smegmatis | 3-methylbutan-2-amine + NAD+ + H2O | - |
? | |
3-methylbutan-2-one + NH3 + NADH + H+ | - |
Cystobacter fuscus | 3-methylbutan-2-amine + NAD+ + H2O | - |
? | |
3-methylbutan-2-one + NH3 + NADH + H+ | - |
Microbacterium sp. MA1 | 3-methylbutan-2-amine + NAD+ + H2O | - |
? | |
3-methylcyclohexanone + NH3 + NADH + H+ | - |
Mycolicibacterium smegmatis | 3-methylcyclohexylamine + NAD+ + H2O | - |
? | |
3-methylcyclohexanone + NH3 + NADH + H+ | - |
Cystobacter fuscus | 3-methylcyclohexylamine + NAD+ + H2O | - |
? | |
3-methylcyclohexanone + NH3 + NADH + H+ | - |
Microbacterium sp. MA1 | 3-methylcyclohexylamine + NAD+ + H2O | - |
? | |
3-methylcyclopentanone + NH3 + NADH + H+ | - |
Mycolicibacterium smegmatis | 3-methylcyclopentylamine + NAD+ + H2O | - |
? | |
3-methylcyclopentanone + NH3 + NADH + H+ | - |
Cystobacter fuscus | 3-methylcyclopentylamine + NAD+ + H2O | - |
? | |
3-methylcyclopentanone + NH3 + NADH + H+ | - |
Microbacterium sp. MA1 | 3-methylcyclopentylamine + NAD+ + H2O | - |
? | |
4-hydroxybutan-2-one + NH3 + NADH + H+ | - |
Mycolicibacterium smegmatis | 3-aminobutan-1-ol + NAD+ + H2O | - |
? | |
4-hydroxybutan-2-one + NH3 + NADH + H+ | - |
Cystobacter fuscus | 3-aminobutan-1-ol + NAD+ + H2O | - |
? | |
4-hydroxybutan-2-one + NH3 + NADH + H+ | - |
Microbacterium sp. MA1 | 3-aminobutan-1-ol + NAD+ + H2O | - |
? | |
4-methylcyclohexanone + NH3 + NADH + H+ | - |
Mycolicibacterium smegmatis | 4-methylcyclohexylamine + NAD+ + H2O | - |
? | |
4-methylcyclohexanone + NH3 + NADH + H+ | - |
Cystobacter fuscus | 4-methylcyclohexylamine + NAD+ + H2O | - |
? | |
4-methylcyclohexanone + NH3 + NADH + H+ | - |
Microbacterium sp. MA1 | 4-methylcyclohexylamine + NAD+ + H2O | - |
? | |
benzaldehyde + NH3 + NADH + H+ | - |
Mycolicibacterium smegmatis | benzylamine + NAD+ + H2O | - |
? | |
benzaldehyde + NH3 + NADH + H+ | - |
Cystobacter fuscus | benzylamine + NAD+ + H2O | - |
? | |
benzaldehyde + NH3 + NADH + H+ | - |
Microbacterium sp. MA1 | benzylamine + NAD+ + H2O | - |
? | |
but-3-en-2-one + NH3 + NADH + H+ | - |
Mycolicibacterium smegmatis | but-3-en-2-amine + NAD+ + H2O | - |
? | |
but-3-en-2-one + NH3 + NADH + H+ | - |
Cystobacter fuscus | but-3-en-2-amine + NAD+ + H2O | - |
? | |
but-3-en-2-one + NH3 + NADH + H+ | - |
Microbacterium sp. MA1 | but-3-en-2-amine + NAD+ + H2O | - |
? | |
cyclohexanone + methylamine + NADH + H+ | - |
Mycolicibacterium smegmatis | N-methylcyclohexylamine + NAD+ + H2O | - |
? | |
cyclohexanone + methylamine + NADH + H+ | - |
Cystobacter fuscus | N-methylcyclohexylamine + NAD+ + H2O | - |
? | |
cyclohexanone + methylamine + NADH + H+ | - |
Microbacterium sp. MA1 | N-methylcyclohexylamine + NAD+ + H2O | - |
? | |
cyclohexanone + NH3 + NADH + H+ | - |
Mycolicibacterium smegmatis | cyclohexylamine + NAD+ + H2O | - |
? | |
cyclohexanone + NH3 + NADH + H+ | - |
Cystobacter fuscus | cyclohexylamine + NAD+ + H2O | - |
? | |
cyclohexanone + NH3 + NADH + H+ | - |
Microbacterium sp. MA1 | cyclohexylamine + NAD+ + H2O | - |
? | |
cyclopentanone + NH3 + NADPH + H+ | - |
Mycolicibacterium smegmatis | cyclopentylamine + NADP+ + H2O | - |
? | |
cyclopentanone + NH3 + NADPH + H+ | - |
Cystobacter fuscus | cyclopentylamine + NADP+ + H2O | - |
? | |
cyclopentanone + NH3 + NADPH + H+ | - |
Microbacterium sp. MA1 | cyclopentylamine + NADP+ + H2O | - |
? | |
hexan-3-one + NH3 + NADH + H+ | - |
Mycolicibacterium smegmatis | hexan-3-amine + NAD+ + H2O | - |
? | |
hexan-3-one + NH3 + NADH + H+ | - |
Cystobacter fuscus | hexan-3-amine + NAD+ + H2O | - |
? | |
hexan-3-one + NH3 + NADH + H+ | - |
Microbacterium sp. MA1 | hexan-3-amine + NAD+ + H2O | - |
? | |
isobutanal + methylamine + NADH + H+ | - |
Mycolicibacterium smegmatis | N,2-dimethylpropan-1-amine + NAD+ + H2O | - |
? | |
isobutanal + methylamine + NADH + H+ | - |
Cystobacter fuscus | N,2-dimethylpropan-1-amine + NAD+ + H2O | - |
? | |
isobutanal + methylamine + NADH + H+ | - |
Microbacterium sp. MA1 | N,2-dimethylpropan-1-amine + NAD+ + H2O | - |
? | |
isobutanal + NH3 + NADH + H+ | - |
Mycolicibacterium smegmatis | 2-methylpropan-1-amine + NAD+ + H2O | - |
? | |
isobutanal + NH3 + NADH + H+ | - |
Cystobacter fuscus | 2-methylpropan-1-amine + NAD+ + H2O | - |
? | |
isobutanal + NH3 + NADH + H+ | - |
Microbacterium sp. MA1 | 2-methylpropan-1-amine + NAD+ + H2O | - |
? | |
pentan-2-one + NH3 + NADH + H+ | - |
Mycolicibacterium smegmatis | pentan-2-amine + NAD+ + H2O | - |
? | |
pentan-2-one + NH3 + NADH + H+ | - |
Cystobacter fuscus | pentan-2-amine + NAD+ + H2O | - |
? | |
pentan-2-one + NH3 + NADH + H+ | - |
Microbacterium sp. MA1 | pentan-2-amine + NAD+ + H2O | - |
? | |
pentan-2-one + NH3 + NADH + H+ | no substrate of wild-type, but substrate of mutants carrying substitution R161M | Petrotoga mobilis | pentan-2-amine + NAD+ + H2O | - |
? | |
pentan-2-one + NH3 + NADH + H+ | no substrate of wild-type, but substrate of mutants carrying substitution R161M | Petrotoga mobilis DSM 10674 | pentan-2-amine + NAD+ + H2O | - |
? | |
pentan-2-one + NH3 + NADH + H+ | - |
Cystobacter fuscus DSM 2262 | pentan-2-amine + NAD+ + H2O | - |
? | |
pentan-3-one + NH3 + NADH + H+ | - |
Mycolicibacterium smegmatis | pentan-3-amine + NAD+ + H2O | - |
? | |
pentan-3-one + NH3 + NADH + H+ | - |
Cystobacter fuscus | pentan-3-amine + NAD+ + H2O | - |
? | |
pentan-3-one + NH3 + NADH + H+ | - |
Microbacterium sp. MA1 | pentan-3-amine + NAD+ + H2O | - |
? | |
pentanal + NH3 + NADH + H+ | - |
Mycolicibacterium smegmatis | n-pentylamine + NAD+ + H2O | - |
? | |
pentanal + NH3 + NADH + H+ | - |
Cystobacter fuscus | n-pentylamine + NAD+ + H2O | - |
? | |
pentanal + NH3 + NADH + H+ | - |
Microbacterium sp. MA1 | n-pentylamine + NAD+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AmDH4 | - |
Petrotoga mobilis |
D187_009359 | - |
Cystobacter fuscus |
D187_009359 | - |
Microbacterium sp. MA1 |
DapB_1 | - |
Mycolicibacterium smegmatis |
Pmob_1166 | - |
Petrotoga mobilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
NH3 | pH 9.5, 23°C | Cystobacter fuscus | |
0.05 | - |
NADPH | pH 9.5, 23°C | Cystobacter fuscus | |
0.1 | - |
NADH | pH 9.5, 23°C | Cystobacter fuscus | |
0.11 | - |
Isobutanal | pH 9.5, 23°C | Cystobacter fuscus | |
0.13 | - |
cyclohexanone | pH 9.5, 23°C | Cystobacter fuscus | |
0.28 | - |
NADPH | pH 9.5, 23°C | Mycolicibacterium smegmatis | |
0.3 | - |
NH3 | pH 9.5, 23°C | Mycolicibacterium smegmatis | |
0.54 | - |
Isobutanal | pH 9.5, 23°C | Mycolicibacterium smegmatis | |
0.58 | - |
NADH | pH 9.5, 23°C | Mycolicibacterium smegmatis | |
0.61 | - |
2-methylcyclohexanone | pH 9.5, 23°C | Mycolicibacterium smegmatis | |
0.94 | - |
cyclohexanone | pH 9.5, 23°C | Mycolicibacterium smegmatis | |
2.5 | - |
(2R)-2-amino-4-oxopentanoate | mutant E102A, pH 9.5, 23°C | Petrotoga mobilis | |
37.8 | - |
NH3 | wild-type, pH 9.5, 23°C | Petrotoga mobilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Petrotoga mobilis | |
NADH | specific activities for both cofactors NADH and NADPH are substrate-dependent | Mycolicibacterium smegmatis | |
NADH | specific activities for both cofactors NADH and NADPH are substrate-dependent | Cystobacter fuscus | |
NADH | specific activities for both cofactors NADH and NADPH are substrate-dependent | Microbacterium sp. MA1 | |
NADPH | specific activities for both cofactors NADH and NADPH are substrate-dependent | Mycolicibacterium smegmatis | |
NADPH | specific activities for both cofactors NADH and NADPH are substrate-dependent | Cystobacter fuscus | |
NADPH | specific activities for both cofactors NADH and NADPH are substrate-dependent | Microbacterium sp. MA1 |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.41 | - |
cyclohexanone | pH 9.5, 23°C | Mycolicibacterium smegmatis | |
0.41 | - |
cyclohexanone | pH 9.5, 23°C | Microbacterium sp. MA1 | |
0.41 | - |
cyclohexanone | pH 9.5, 23°C | Petrotoga mobilis | |
1.99 | - |
cyclohexanone | pH 9.5, 23°C | Cystobacter fuscus | |
2.76 | - |
Isobutanal | pH 9.5, 23°C | Mycolicibacterium smegmatis | |
2.76 | - |
Isobutanal | pH 9.5, 23°C | Microbacterium sp. MA1 | |
2.76 | - |
Isobutanal | pH 9.5, 23°C | Petrotoga mobilis | |
4.94 | - |
2-methylcyclohexanone | pH 9.5, 23°C | Mycolicibacterium smegmatis | |
4.94 | - |
2-methylcyclohexanone | pH 9.5, 23°C | Microbacterium sp. MA1 | |
4.94 | - |
2-methylcyclohexanone | pH 9.5, 23°C | Petrotoga mobilis | |
10623 | - |
NH3 | pH 9.5, 23°C | Cystobacter fuscus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
NH3 | pH 9.5, 23°C | Cystobacter fuscus | |
0.0008 | - |
NH3 | pH 9.5, 23°C | Mycolicibacterium smegmatis | |
0.054 | - |
cyclohexanone | pH 9.5, 23°C | Cystobacter fuscus | |
0.118 | - |
NH3 | wild-type, pH 9.5, 23°C | Petrotoga mobilis | |
0.209 | - |
Isobutanal | pH 9.5, 23°C | Cystobacter fuscus | |
0.26 | - |
2-methylcyclohexanone | pH 9.5, 23°C | Mycolicibacterium smegmatis | |
0.62 | - |
Isobutanal | pH 9.5, 23°C | Mycolicibacterium smegmatis | |
1.705 | - |
NADH | pH 9.5, 23°C | Cystobacter fuscus | |
1.72 | - |
cyclohexanone | pH 9.5, 23°C | Mycolicibacterium smegmatis | |
1.99 | - |
NADH | pH 9.5, 23°C | Mycolicibacterium smegmatis | |
2.81 | - |
(2R)-2-amino-4-oxopentanoate | mutant E102A, pH 9.5, 23°C | Petrotoga mobilis | |
4.537 | - |
NADPH | pH 9.5, 23°C | Cystobacter fuscus | |
4.89 | - |
NADPH | pH 9.5, 23°C | Mycolicibacterium smegmatis | |
130 | - |
(2R)-2-amino-4-oxopentanoate | wild-type, pH 9.5, 23°C | Petrotoga mobilis | |
1440 | - |
NADH | wild-type, pH 9.5, 23°C | Petrotoga mobilis |