Protein Variants | Comment | Organism |
---|---|---|
K66Q/S149G/N262C | the mutant shows activity with phenylacetone and 4-phenyl-2-butanone | Rhodococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
4-phenyl-2-butanone | mutant enzyme K66Q/S149G/N262C, at pH 9.6 and 30°C | Rhodococcus sp. | |
4 | - |
phenylacetone | mutant enzyme K66Q/S149G/N262C, at pH 9.6 and 30°C | Rhodococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + H2O + NAD+ | Rhodococcus sp. | - |
phenylpyruvate + NH3 + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus sp. | Q59771 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-phenyl-2-butanone + NH3 + NADH + H+ | substrate for mutant enzyme K66Q/S149G/N262C | Rhodococcus sp. | (R)-1-methyl-3-phenylpropylamine + H2O + NAD+ | - |
? | |
L-phenylalanine + H2O + NAD+ | - |
Rhodococcus sp. | phenylpyruvate + NH3 + NADH + H+ | - |
? | |
phenylacetone + NH3 + NADH + H+ | substrate for mutant enzyme K66Q/S149G/N262C | Rhodococcus sp. | (R)-amphetamine + H2O + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PheDH | - |
Rhodococcus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
phenylacetone | mutant enzyme K66Q/S149G/N262C, at pH 9.6 and 30°C | Rhodococcus sp. | |
0.72 | - |
4-phenyl-2-butanone | mutant enzyme K66Q/S149G/N262C, at pH 9.6 and 30°C | Rhodococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Rhodococcus sp. |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.18 | - |
phenylacetone | mutant enzyme K66Q/S149G/N262C, at pH 9.6 and 30°C | Rhodococcus sp. | |
0.5 | - |
4-phenyl-2-butanone | mutant enzyme K66Q/S149G/N262C, at pH 9.6 and 30°C | Rhodococcus sp. |