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Literature summary for 1.4.1.2 extracted from

  • Xian, L.; Zhang, Y.; Cao, Y.; Wan, T.; Gong, Y.; Dai, C.; Ochieng, W.A.; Nasimiyu, A.T.; Li, W.; Liu, F.
    Glutamate dehydrogenase plays an important role in ammonium detoxification by submerged macrophytes (2020), Sci. Total Environ., 722, 137859 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
NH4+ the activity of glutamate dehydrogenase (GDH) in the ammonium-tolerant species Myriophyllum spicatum leaves performs a dose-response curve with increase of 169% for NADH-dependent GDH with the [NH4+-N] increasing from 0 to 100 mg/l, while glutamine synthetase (GS) activity slightly changes. Compared to that, for the ammonium-sensitive species, Potamogeton lucens, the activity of GDH records no major changes, while the GS increases slightly (17%) Myriophyllum spicatum
NH4+ the activity of glutamate dehydrogenase (GDH) in the ammonium-tolerant species Myriophyllum spicatum leaves performs a dose-response curve with increase of 169% for NADH-dependent GDH with the [NH4+-N] increasing from 0 to 100 mg/l, while glutamine synthetase (GS) activity slightly changes. Compared to that, for the ammonium-sensitive species, Potamogeton lucens, the activity of GDH records no major changes, while the GS increases slightly (17%) Potamogeton lucens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ required Myriophyllum spicatum
Ca2+ required Potamogeton lucens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + H2O + NAD+ Myriophyllum spicatum
-
2-oxoglutarate + NH3 + NADH + H+
-
r
L-glutamate + H2O + NAD+ Potamogeton lucens
-
2-oxoglutarate + NH3 + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Myriophyllum spicatum
-
-
-
Myriophyllum spicatum
-
collected from Erhai Lake, a freshwater lake on the Yunnan-Guizhou Plateau, southwest China, ammonium-tolerant species
-
Potamogeton lucens
-
ammonium-sensitive species
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Myriophyllum spicatum
-
leaf
-
Potamogeton lucens
-
shoot
-
Myriophyllum spicatum
-
shoot
-
Potamogeton lucens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0495 0.133 pH 8.2, 30°C Myriophyllum spicatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD+
-
Myriophyllum spicatum 2-oxoglutarate + NH3 + NADH + H+
-
r
L-glutamate + H2O + NAD+
-
Potamogeton lucens 2-oxoglutarate + NH3 + NADH + H+
-
r

Synonyms

Synonyms Comment Organism
NADH-dependent GDH
-
Myriophyllum spicatum
NADH-dependent GDH
-
Potamogeton lucens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
reductive amination reaction, assay at Myriophyllum spicatum
30
-
reductive amination reaction, assay at Potamogeton lucens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.2
-
reductive amination reaction, assay at Myriophyllum spicatum
8.2
-
reductive amination reaction, assay at Potamogeton lucens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Myriophyllum spicatum
NAD+
-
Potamogeton lucens
NADH
-
Myriophyllum spicatum
NADH
-
Potamogeton lucens

General Information

General Information Comment Organism
physiological function in practical water restoration by aquatic plants, the alternative pathway of GDH is more important than the pathway catalyzed by GS in determining the tolerance of submerged macrophytes to high ammonium concentration. Both NADH-dependent and NADPH-dependent GDH (EC 1.4.1.4) show species-dependent variation, in the ammonium-tolerant species, Myriophyllum spicatum, there is a dose-response curve (from 49.46 to 132.99 nmol/min/mg protein for NADH-dependent GDH and 28.98 to 58.67 nmol/min/mg protein for NADPH-GDH), but in the ammonium-sensitive species, Potamogeton lucens, there is little change in activity Myriophyllum spicatum
physiological function in practical water restoration by aquatic plants, the alternative pathway of GDH is more important than the pathway catalyzed by GS in determining the tolerance of submerged macrophytes to high ammonium concentration. Both NADH-dependent and NADPH-dependent GDH (EC 1.4.1.4) show species-dependent variation, in the ammonium-tolerant species, Myriophyllum spicatum, there is a dose-response curve (from 49.46 to 132.99 nmol/min/mg protein for NADH-dependent GDH and 28.98 to 58.67 nmol/min/mg protein for NADPH-GDH), but in the ammonium-sensitive species, Potamogeton lucens, there is little change in activity Potamogeton lucens
physiological function the activity of glutamate dehydrogenase in the ammonium-tolerant species Myriophyllum spicatum leaves increases 169% for NADH-dependent GDH and 103% for NADPH-dependent GDH with the [NH4+-N] increasing from 0 to 100 mg/l, performing a dose-response curve while glutamine synthetase activity slightly changes Myriophyllum spicatum