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Literature summary for 1.4.1.2 extracted from

  • Fontaine, J.X.; Terce-Laforgue, T.; Armengaud, P.; Clement, G.; Renou, J.P.; Pelletier, S.; Catterou, M.; Azzopardi, M.; Gibon, Y.; Lea, P.J.; Hirel, B.; Dubois, F.
    Characterization of a NADH-dependent glutamate dehydrogenase mutant of Arabidopsis demonstrates the key role of this enzyme in root carbon and nitrogen metabolism (2012), Plant Cell, 24, 4044-4065.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information construction of backcrossed homozygous gdh1, gdh2, gdh3, gdh1-2, and gdh1-2-3 mutants. Enzyme activity in the roots of the gdh1 single mutant is similar to the wild-type, NADH-GDH activity in the roots of the gdh2 mutant is about 25% lower than the wild type, whereas in the roots of the gdh3 mutant, the enzyme activity is 30% higher. In the leaves, there is a 60% reduction in NADH-GDH activity in the gdh1 single mutant but not in the other two single mutants. By contrast, both in the roots and in the leaves of the gdh1-2 double mutant, a dramatic decrease in NADH-GDH activity occurs, butin the gdh1-2 double mutant, some remaining enzyme activity is still detected in the roots. No NADH-GDH enzyme activity is detected in either of the organs of the gdh1-2-3 triple mutant. No NADPH-GDH enzyme activity is detected in the wild type or in the gdh single, double, or triple mutants. Metabolic profiling of the gdh1-2-3 triple mutant, e.g. placed under continuous darkness, overview Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Arabidopsis thaliana 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate + H2O + NAD+ Arabidopsis thaliana
-
2-oxoglutarate + NH3 + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
three genes GDH1, GDH2, and GDH3 encoding the enzyme subunits
-

Source Tissue

Source Tissue Comment Organism Textmining
companion cell all GDH genes are expressed in the mitochondria of the root companion cells Arabidopsis thaliana
-
leaf
-
Arabidopsis thaliana
-
additional information in the wild-type, the specific activity of NADH-GDH is over 10times higher in the roots compared with that measured in the leaves Arabidopsis thaliana
-
root DH3 is only active in roots Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate + H2O + NAD+
-
Arabidopsis thaliana 2-oxoglutarate + NH3 + NADH + H+
-
r

Synonyms

Synonyms Comment Organism
NADH-dependent glutamate dehydrogenase
-
Arabidopsis thaliana
NADH-GDH
-
Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Arabidopsis thaliana
NADH
-
Arabidopsis thaliana

General Information

General Information Comment Organism
malfunction a gdh1-2-3 triple mutant exhibits major differences to the wild-type in gene transcription and metabolite concentrations, and these differences appear to originate in the roots, metabolic profile of the gdh1-2-3 triple mutant, overview Arabidopsis thaliana
physiological function the main physiological function of NADH-GDH is to provide 2-oxoglutarate for the tricarboxylic acid cycle. Differences in key metabolites of the tricarboxylic acid cycle in the triple mutant versus the wild-type indicate that, through metabolic processes operating mainly in roots, there is a strong impact on amino acid accumulation, in particular alanine, gamma-aminobutyrate, and aspartate in both roots and leaves, phenotypes, overview Arabidopsis thaliana