Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of backcrossed homozygous gdh1, gdh2, gdh3, gdh1-2, and gdh1-2-3 mutants. Enzyme activity in the roots of the gdh1 single mutant is similar to the wild-type, NADH-GDH activity in the roots of the gdh2 mutant is about 25% lower than the wild type, whereas in the roots of the gdh3 mutant, the enzyme activity is 30% higher. In the leaves, there is a 60% reduction in NADH-GDH activity in the gdh1 single mutant but not in the other two single mutants. By contrast, both in the roots and in the leaves of the gdh1-2 double mutant, a dramatic decrease in NADH-GDH activity occurs, butin the gdh1-2 double mutant, some remaining enzyme activity is still detected in the roots. No NADH-GDH enzyme activity is detected in either of the organs of the gdh1-2-3 triple mutant. No NADPH-GDH enzyme activity is detected in the wild type or in the gdh single, double, or triple mutants. Metabolic profiling of the gdh1-2-3 triple mutant, e.g. placed under continuous darkness, overview | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | Arabidopsis thaliana | - |
2-oxoglutarate + NH3 + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
three genes GDH1, GDH2, and GDH3 encoding the enzyme subunits | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
companion cell | all GDH genes are expressed in the mitochondria of the root companion cells | Arabidopsis thaliana | - |
leaf | - |
Arabidopsis thaliana | - |
additional information | in the wild-type, the specific activity of NADH-GDH is over 10times higher in the roots compared with that measured in the leaves | Arabidopsis thaliana | - |
root | DH3 is only active in roots | Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | - |
Arabidopsis thaliana | 2-oxoglutarate + NH3 + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
NADH-dependent glutamate dehydrogenase | - |
Arabidopsis thaliana |
NADH-GDH | - |
Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Arabidopsis thaliana | |
NADH | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | a gdh1-2-3 triple mutant exhibits major differences to the wild-type in gene transcription and metabolite concentrations, and these differences appear to originate in the roots, metabolic profile of the gdh1-2-3 triple mutant, overview | Arabidopsis thaliana |
physiological function | the main physiological function of NADH-GDH is to provide 2-oxoglutarate for the tricarboxylic acid cycle. Differences in key metabolites of the tricarboxylic acid cycle in the triple mutant versus the wild-type indicate that, through metabolic processes operating mainly in roots, there is a strong impact on amino acid accumulation, in particular alanine, gamma-aminobutyrate, and aspartate in both roots and leaves, phenotypes, overview | Arabidopsis thaliana |