Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-aspartate | catalytic activator, 2fold activation at 0.1 mM, effect on enzyme kinetics, overview | Janthinobacterium lividum |
Cloned (Comment) | Organism |
---|---|
recombinant expression in Escherichia coli TOP10 cells | Janthinobacterium lividum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | substrate inhibition | Janthinobacterium lividum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the enzyme shows positive cooperativity towards 2-oxoglutarate and NADH, and Michaelis-Menten type kinetics with ammonium chloride in the absence of catalytic activator L-aspartate. L-aspartate effect on enzyme kinetics, overview | Janthinobacterium lividum | |
0.041 | - |
2-oxoglutarate | pH 6.5, 25°C, recombinant enzyme, in presence of 10 mM L-aspartate | Janthinobacterium lividum | |
33.9 | - |
NH3 | as NH4+, pH 6.5, 25°C, recombinant enzyme, in absence of L-aspartate | Janthinobacterium lividum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | Janthinobacterium lividum | - |
2-oxoglutarate + NH3 + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Janthinobacterium lividum | A5LH94 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli TOP10 cells by hydrophobic interaction chromatography | Janthinobacterium lividum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate + H2O + NAD+ | - |
Janthinobacterium lividum | 2-oxoglutarate + NH3 + NADH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
NAD-dependent glutamate dehydrogenase | - |
Janthinobacterium lividum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
reductive amination assay at | Janthinobacterium lividum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
reductive amination assay at | Janthinobacterium lividum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Janthinobacterium lividum | |
NADH | - |
Janthinobacterium lividum |