Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Clostridium tetani |
Crystallization (Comment) | Organism |
---|---|
apo form and in complex with meso-diaminopimelate, D-leucine, and 4-methyl-2-oxopentanoic acid, sitting drop vapor diffusion method, using MES (0.1 M, pH 6.0), 15% (w/v) PEG 20000, and 5% (v/v) glycerol | Clostridium tetani |
Protein Variants | Comment | Organism |
---|---|---|
Q154L/T173I/R199M/P248S/H249N/N276S | the mutant shows strongly reduced deamination activity with meso-2,6-diaminoheptanedioate and increased animation activity with pyruvate, phenylpyruvate, and 4-methyl-2-oxopentanoic acid compared to the wild type enzyme | Clostridium tetani |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
meso-2,6-diaminoheptanedioate + H2O + NADP+ | Clostridium tetani | - |
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+ | - |
r | |
meso-2,6-diaminoheptanedioate + H2O + NADP+ | Clostridium tetani E88 | - |
L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium tetani | Q890V3 | - |
- |
Clostridium tetani E88 | Q890V3 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, and Superdex 200 gel filtration | Clostridium tetani |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.022 | - |
with 4-methyl-2-oxopentanoic acid as substrate, at pH 9.0 and 30°C | Clostridium tetani |
0.219 | - |
with pyruvate as substrate, at pH 9.0 and 30°C | Clostridium tetani |
1.036 | - |
with meso-2,6-diaminoheptanedioate as substrate, at pH 9.5 and 30°C | Clostridium tetani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methyl-2-oxopentanoic acid + NH3 + NADPH + H+ | - |
Clostridium tetani | ? | - |
? | |
4-methyl-2-oxopentanoic acid + NH3 + NADPH + H+ | - |
Clostridium tetani E88 | ? | - |
? | |
meso-2,6-diaminoheptanedioate + H2O + NADP+ | - |
Clostridium tetani | L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+ | - |
r | |
meso-2,6-diaminoheptanedioate + H2O + NADP+ | - |
Clostridium tetani E88 | L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+ | - |
r | |
additional information | no activity with phenylpyruvate | Clostridium tetani | ? | - |
? | |
additional information | no activity with phenylpyruvate | Clostridium tetani E88 | ? | - |
? | |
pyruvate + NADPH | - |
Clostridium tetani | ? | - |
? | |
pyruvate + NADPH | - |
Clostridium tetani E88 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DAPDH | - |
Clostridium tetani |
meso-diaminopimelate dehydrogenase | - |
Clostridium tetani |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Clostridium tetani |