Literature summary for 1.4.1.16 extracted from

Akita, H.; Seto, T.; Ohshima, T.; Sakuraba, H.
Structural insight into the thermostable NADP+-dependent meso-diaminopimelate dehydrogenase from Ureibacillus thermosphaericus (2015), Acta Crystallogr. Sect. D, 71, 1136-1146 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli Rosetta (DE3) cells	Ureibacillus thermosphaericus

Crystallization (Commentary)

Crystallization (Comment)	Organism
apo form and in complex with NADP+ and N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid, sitting drop vapor diffusion method, using 1.9 M ammonium sulfate,0.1 M Tris-HCl pH 7.5	Ureibacillus thermosphaericus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
meso-2,6-diaminoheptanedioate + H2O + NADP+	Ureibacillus thermosphaericus	-	L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+	-	r

Organism

Organism	UniProt	Comment	Textmining
Ureibacillus thermosphaericus	G1UII1	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni+-charged chelating Sepharose column chromatography and Toyopearl butyl-650M column chromatography	Ureibacillus thermosphaericus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
meso-2,6-diaminoheptanedioate + H2O + NADP+	-	Ureibacillus thermosphaericus	L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+	-	r

Subunits

Subunits	Comment	Organism
homodimer	x-ray crystallography	Ureibacillus thermosphaericus

Synonyms

Synonyms	Comment	Organism
DAPDH	-	Ureibacillus thermosphaericus
NADP+-dependent meso-diaminopimelate dehydrogenase	-	Ureibacillus thermosphaericus

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Ureibacillus thermosphaericus

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Release 2023.1 (January 2023)