General Stability | Organism |
---|---|
highly labile enzyme activity | Medicago sativa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azaserine | glutamine antagonist, completely inhibited by 10 mM | Medicago sativa | |
citrate | inhibited by high concentrations, 40 mM | Medicago sativa | |
L-glutamate | - |
Medicago sativa | |
malate | inhibited by high concentrations, 40 mM | Medicago sativa | |
additional information | not inhibited by 2,2-dipyridyl or N-ethylmaleimide | Medicago sativa | |
NAD+ | - |
Medicago sativa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0042 | - |
NADH | - |
Medicago sativa | |
0.033 | - |
2-oxoglutarate | - |
Medicago sativa | |
0.466 | - |
L-glutamine | - |
Medicago sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
200000 | - |
1 * 200000, SDS-PAGE | Medicago sativa |
235000 | - |
gel filtration | Medicago sativa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + NADH | Medicago sativa | key enzyme in pathway of assimilation of symbiotically fixed N2 into amino acids | glutamate + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Medicago sativa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
208fold purification | Medicago sativa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root nodule | high enzyme activity in nodules compared to roots and other organs | Medicago sativa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.1 | - |
- |
Medicago sativa |
Storage Stability | Organism |
---|---|
-80°C, 50% glycerol, 100 mM potassium phosphate, 3 mM DTT, stable for up to 1 month | Medicago sativa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 2-oxoglutarate + NADH | - |
Medicago sativa | L-glutamate + NAD+ | - |
? | |
L-glutamine + 2-oxoglutarate + NADH | key enzyme in pathway of assimilation of symbiotically fixed N2 into amino acids | Medicago sativa | glutamate + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 200000, SDS-PAGE | Medicago sativa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Medicago sativa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | below and above activity declines rapidly | Medicago sativa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Medicago sativa |